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Gene expression analysis of RNAs isolated from TPEN-untreated and TPEN-treated human keratinocytes

ABSTRACT: In order to identify the zinc responsive genes, we performed a gene expression microarray analysis for the RNAs isolated from TPEN-untreated and TPEN-treated human keratinocytes

ORGANISM(S): Homo sapiens

PROVIDER: GSE99204 | GEO | 2017/10/05

SECONDARY ACCESSION(S): PRJNA387554

REPOSITORIES: GEO

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Project description:DNA microarrays were conducted on E. coli K12 cells stressed with 10 μM in N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN). Overall, 260 genes varied in expression, 114 up-regulated and 146 down-regulated by Zn deprivation Keywords: TPEN stress Array hybridizations were carried out for three RNA samples prepared from three independent cultures (control or TPEN-treated).

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Project description:Gene expression analysis of RNAs isolated from TSA-untreated and TSA-treated human keratinocytes

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Project description:Gene expression analysis of RNAs isolated from C646-untreated and C646-treated human keratinocytes

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Homo sapiens

Project description:Gene expression analysis of RNAs isolated from TPEN-untreated and TPEN-treated human keratinocytes

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Next Generation Sequencing Facilitates Quantitative Analysis of UVB(20mJ/cm2 and 40mJ/cm2) exposed and untreated HaCaT keratinocytes Transcriptomes

Project description:Purpose: To compare the transcriptomes of UVB (20mJ/cm2 and 40mJ/cm2) exposed and untreated HaCaT keratinocytes by RNA-Seq analysis, trying to find differences in gene expression between UVB exposed and untreated of keratinocytes and then elucidate the candidate genes that may play important roles in the differentiation of UVB-induced damage in keratinocytes. Methods: HaCaT keratinocytes were subjected to 20mJ/cm2 and 40mJ/cm2 UVB irradiation. Results: To better understand the effects of UVB (20 mJ/cm2), mRNA-sequecing (n=3) were completed by Novogene Inc. A total 891 differentially expressed genes (DEGs) were identified between UV group and control group with 604 down-regulated and 287 up-regulated. A total of 4036 differentially expressed genes (DEGs) which compared with untreated group were identified by RNA-Seq, which provided abundant data for further analysis.

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