Robust detection of chromosomal contacts from small cell numbers using low-input Capture-C

ABSTRACT: Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This hampers the investigation of chromatin structure in rare cell populations. We present two new low-input Capture-C protocols that generate high-quality, reproducible interaction profiles from fewer than 20,000 cells, and show that these are not biased by PCR amplification or the degree of formaldehyde fixation.

ORGANISM(S): Mus musculus

PROVIDER: GSE99257 | GEO | 2017/11/20

SECONDARY ACCESSION(S): PRJNA387734

REPOSITORIES: GEO