Genomics

Dataset Information

37

A class of GATA3 mutation reprograms the breast cancer transcriptional network through gain and loss of function


ABSTRACT: A pioneer transcription factor, GATA3, is one of the most frequently mutated genes in breast cancer, yet the impact of these mutations is largely unknown. We generated a GATA3 mutant cell line (T47D wt/R330fs) by CRISPR. Mutation of one allele of GATA3 led to loss of binding and decreased expression at a subset of genes, including Progesterone Receptor. At other loci, associated with epithelial to mesenchymal transition, gain of binding at a novel sequence motif correlated with increased gene expression. Our results illuminate tumor-promoting functions of specific GATA3 mutations in breast cancer. Overall design: Genome-wide mapping of chromatin localization of luminal transcription factors in GATA3 mutant cells

INSTRUMENT(S): Illumina HiSeq 2000 (Homo sapiens)

SUBMITTER: Paul A Wade  

PROVIDER: GSE99479 | GEO | 2017-10-16

SECONDARY ACCESSION(S): PRJNA388581

REPOSITORIES: GEO

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