Project description:Direct comparison of FACS sorted EGFP positive brain macrophages vs pooled Total Brain extract Keywords: Direct pairwise comparison
Project description:Direct comparison of FACS sorted EGFP positive renal macrophages vs pooled Total Kidney extract Keywords: Direct pairwise comparison
Project description:Use CEN quechers method to extract 2.5g honey and use PSA to clean up matrix. Using ACN-water-0.01%HCOOH for compound separation and HRMS analysis with Thermo Orbitrap Exploris 120. Data was acquired in Full scan-ddms2 mode. This included a full scan over the m/z range 100- 1000 at full width at half maximum (FWHM) resolution of 60,000, and a data-dependent-MS2 scan at FWHM resolution of 15,000 on the top 4 ions. The ionization was performed in positive ESI with an inlusion list collated from OPPIN website, and to gain more information about fragment ions in the QC sample, we use an Automated Exclusion List Generation workflow, so one QC sample finally gave 3 injections.
Project description:Wild type and alkbh1a/1b seeds were stratified for two days, dry and stratified seeds were collected to extract total RNA for mRNA-seq. We then performed gene expression profiling analysis using data obtained from mRNA-seq.
Project description:Wild type and atdamt1 seedlings were heat treated for 2.5 hours, control and treated seedlings were collected to extract total RNA for mRNA-seq. We then performed gene expression profiling analysis using data obtained from mRNA-seq.