Project description:The submitted dataset contains multiomic (single-nucleus RNA-seq and scATAC-seq) data from adult (p56) mouse male and female lumbar spinal cords for cross-species analysis.
Project description:Analysis of the combined dataset of normal skin, actinic keratosis and cutaneous squamous cell carcinoma (cSCC) previously submitted in (GSE45216) at genomic/transciptomic level. We identified potential cSCC driver genes significantly mutated and upregulated in cSCC relative to normal skin.
Project description:The intermediate filament protein Nestin serves as a biomarker for stem cells and has been used to identify subsets of cancer stem-like cells. However, the mechanistic contributions of Nestin to cancer pathogenesis are not understood. Here we report that Nestin binds the hedgehog pathway transcription factor Gli3 to mediate the development of medulloblastomas of the hedgehog subtype. In a mouse model system, Nestin levels increased progressively during medulloblastoma formation resulting in enhanced tumor growth. Conversely, loss of Nestin dramatically inhibited proliferation and promoted differentiation. Mechanistic investigations revealed that the tumor-promoting effects of Nestin were mediated by binding to Gli3, a zinc finger transcription factor that negatively regulates hedgehog signaling. Nestin binding to Gli3 blocked Gli3 phosphorylation and its subsequent proteolytic processing, thereby abrogating its ability to negatively regulate the hedgehog pathway. Our findings show how Nestin drives hedgehog pathway-driven cancers and uncover in Gli3 a therapeutic target to treat these malignancies.
Project description:We previously deposited a similar batch of data to the Gene Expression Omnibus (GEO) database under the accession code GSE232332. This dataset was to be a further validation of that study. Unlike the previous dataset, this has not, however, been submitted for publication. This dataset includes epigenetic data form 23 patients with invasive oesophageal adenocarcinoma, 25 with intramucosal oesophageal adenocarcinoma, 35 patients with non-dysplastic Barrett's esophagus and 32 controls who did not have Barrett's esophagus or cancer at endoscopy, performed within 2 weeks of the saliva sample which was obtained after fasting for at least 1 hour.