Project description:Interlab Study of LC-MS/MS analyis of Marine Dissolved Organic Matter from SIO Pietr (San Diego, California, USA) and algae extracts, extracted via PPL SPE
Project description:The deletion of TCF19 in CAL27,oral squamous cell carcinoma cell line,was conducted by using CRISPR/Cas9 system. For WT and TCF19 KO CAL27 cell lines, samples were done in triplicates. A total of 3μg of high-quality RNA per sample was used for ribosomal RNA removal by the Epicentre Ribo-zero rRNA Removal Kit (Epicentre, USA) and the sequencing library was prepared using the rRNA-depleted RNA by the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina, San Diego, CA, USA), followed by the 150-bp paired-end sequencing on the HiSeq X Ten instrument (Illumina, San Diego, CA, USA) according to the manufacturer’s protocols.
Project description:Environmental extracts and fractions obtained with passive sampling (HP-20, SMIRC) from Mission Bay, Point Loma, Scripps Pier, Salton Sea (San Diego and Imperial Counties)
Project description:Interlab Study of LC-MS/MS analyis of Marine Dissolved Organic Matter from SIO Pietr (San Diego, California, USA) and algae extracts, extracted via PPL SPE
Project description:To identify microRNA changes during plasmacytoid dendritic cell (PDC) activation, we stimulated human primary PDCs with 10ug/ml R837 (Invivogen, San Diego, CA, USA) for 4 hours.
Project description:EV RNA samples from MH-S cells were prepared for small RNA sequencing by TruSeq Small RNA Sample Prep Kits (Illumina, San Diego,USA), using a minimum of 1 μg RNA per sample.
Project description:In this study, 56 semen samples were collected from healthy Korean males aged 18 to 70 and data was obtained utilizing the Illumina Infinium MethylationEPIC BeadChip array (Illumina, San Diego, CA, USA) at Macrogen Inc. (Macrogen, Seoul, Korea).