Project description:Data set contains LC-MS/MS data acquired in DD mode on 201 extracts of regular polymer swabs used for 16S sequencing. 90% EtOH extractions. The data are for plant eaters who consume more than 30 types of plants vs. those who do not.
Project description:Data set contains LC-MS/MS data acquired in DD mode on 489 extracts of regular polymer swabs used for 16S sequencing. 90% EtOH extractions. The dataset contains data for subjects who reported no antibiotic use within a year vs. those who used them within last month.
Project description:Analysis of COVID-19 hospitalized patients, with different kind of symptoms, by human rectal swabs collection and 16S sequencing approach.
Project description:<p>The focus of this study was to better understand the effects of cigarette smoking on the vaginal microbiome. There were two phases of the study, cross-sectional and longitudinal, conducted at the Center for Health Behavior Research at the University of Maryland School of Public Health. In the cross-sectional phase, 20 smokers and 20 non-smokers collected mid-vaginal swabs, measured their vaginal pH, prepared a vaginal smear on a slide for Nugent Gram stain analysis, and completed questionnaires about demographics, tobacco use, and reproductive and sexual health history. Smoking status was confirmed through self-report, carbon monoxide exhalation and saliva cotinine measures. Secretions from the mid-vaginal swabs were tested for presence/absence of HPV strains and GC-MS was used to quantify the levels of over 600 metabolites.</p> <p>In the longitudinal phase, 7 participants who were current smokers and motivated to quit smoking were recruited and followed for up to 12 weeks. On a daily basis, participants collected mid-vaginal swabs, measured their vaginal pH, and prepared a mid-vaginal smear on a slide for Nugent Gram stain analysis, and completed daily diaries on tobacco use and reproductive health. Carbon monoxide exhalation and saliva cotinine measures were collected at weekly clinical visits. In addition, participants had weekly behavioral counseling sessions about smoking cessation and used Nicoderm CQ patches to aid in quitting smoking. The self-collected vaginal swabs were used for DNA extractions,16s rRNA sequencing and measurement of metabolites in vaginal fluid.</p>
Project description:Twenty-seven (n=27) Piper species. Leaf tissues extracted with EtOH/Water (75:25) and profiled with LC-HRMS in dd-MS2 acquisition mode.
Project description:Leaf tissue from twenty-seven (n=27) Piperaceae plants extracted with EtOH/Water (75:25) and profiled with LC-IM-MS in dd-MS2 acquisition mode.
Project description:The Arabidopsis cytochrome P450 KLUH (KLU)/CYP78A5 promotes organ growth in a non-cell autonomous manner. To identify genes regulated by KLU activity, homozygous klu-2 mutants carrying constructs for EtOH-inducible overexpression of wild-type KLU (35S::AlcR-AlcA::KLU) or of enzymatically inactive KLU protein (35S::AlcR-AlcA::KLUmut) were induced with EtOH and sampled at 90 min and 240 min after induction for gene expression changes. Keywords: Time course, genetic modification
2007-12-04 | GSE9201 | GEO
Project description:16S Sequencing from Equine Uterine Swabs