Project description:We report that a transcriptional regulator that originated in the lineage that gave rise to multiple host-associated Candida species is a key component of the circuitry that governs the C. albicans cell surface composition. Specifically, we show that the transcription regulator ZCF21 controls the expression of genes encoding multiple cell surface proteins and cell wall modifying enzymes. Transcriptome (RNA-seq) analysis of 2 Candida albicans strains (reference strain and zcf21 deletion mutant) grown under 4 culture conditions (YPD broth at 30°C for 24h; YPD broth at 30°C in the presence of 15 mM caffeine for 24h; Todd-Hewitt agar at 37°C for 24h; Todd-Hewitt agar at 37°C for 24h in the presence of 5 mM caffeine).
Project description:Total RNA was isolated from mid-log phase Streptococcus agalactiae 874391 wild-type cells grown in Todd-Hewitt broth (THB) medium and sequenced using Illumina NextSeq500
Project description:Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in SczA (∆sczA strain GU2791), grown in Todd-Hewitt broth (THB) medium and sequenced using Illumina NextSeq500.
Project description:Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in CopY (∆copY strain GU2857), grown in Todd-Hewitt broth (THB) medium and sequenced using Illumina NextSeq500.