Project description:Samples of hair follicles extracts. The study combined targeted and untargeted analysis to detect propimycin cyclic peptide, a potential antibiotic produced by the Propionibacterium acnes in skin pores. The untargeted analysis did not allow for the detection, while targeted using QqQ instrument, did.
Project description:We developed a novel strategy using integrated DDA-PRM-dMRM to comprehensively profile and quantify high-risk HCPs in CHO cell-derived biotherapeutics. Firstly, we performed deep proteomic analysis of CHO cells using DDA-PASEF mode, constructing a high-quality spectral library covering all potential HCPs. Based on the constructed library, we validated the target list using PRM-PASEF and MRM modes.
Project description:Latrophilin 2 (ADGRL2), an adhesion GPCR, mediates signaling through its intracellular loop 3 via a unique QQQ binding patch within the Gα13 GTPase domain, playing a critical role in the regulation of normal epidermal differentiation.
Project description:The study investigated presentation of HLA A2 restricted H3.3K27M neopeptide using immunopeptidomics followed by DDA and/or targeted multiple monitoring reaction (MRM).
Project description:We profiled the transcriptomes of latency-competent cells derived from the human cancer cell lines H2087 (lung adenocarcinoma) and HCC1954 (breast adenocarcinoma) in mitogen-rich and mitogen-low media (MRM and MLM, respectively). In addition, we analyzed the epigenetic landscape of these cell lines under MLM conditions.
2016-03-29 | GSE72956 | GEO
Project description:Detection of rare plasmid hosts using a targeted Hi-C approach