Project description:The effect of a long-term calorie restricted diet was evaluated in PBMCs of rhesus monkeys For biopsy collection, the animals were given buprenorphine (0.01-0.03 mg/kg, IM) in the morning immediately preceding all biopsies and again that afternoon
Project description:The effect of a long-term calorie restricted diet was evaluated in vastus lateralis muscle of rhesus monkeys For biopsy collection, the animals were given buprenorphine (0.01-0.03 mg/kg, IM) in the morning immediately preceding all biopsies and again that afternoon
Project description:Differentiated somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells by forced expression of four transcription factors—Oct4, Sox2, Klf4, and c-Myc. However, it remains undetermined whether the reprogrammed iPS cells are fully pluripotent, resembling normal embryonic stem (ES) cells, given that no iPS cell lines have been shown to possess the capability to autonomously generate full-term mice after injection into tetraploid blastocysts. Here, we provide evidence demonstrating that iPS cells induced by the four transcription factors can be fully pluripotent and that full-term mice can be produced from complemented tetraploid blastocysts. This work serves as a proof of principle that iPS cells can generate full term embryos by tetraploid complementation.