Project description:Investigation of whole genome gene expression level changes over time of Fusarium verticillioides wild-type in liquid fumonisin inducing media (GYAM) Fusarium verticillioides produces a polyketide derived mycotoxin, fumonsin, over time in liquid media, Proctor et al, Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis, Fungal Genetics and Biology, 38:237-249
Project description:Investigation of whole genome gene expression level changes over time of Fusarium verticillioides wild-type in liquid fumonisin inducing media (GYAM). Fusarium verticillioides produces a polyketide derived mycotoxin, fumonsin, over time in liquid media (Proctor et al, Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis, Fungal Genetics and Biology, 38:237-249).
Project description:Investigation of whole genome gene expression level changes after 6 days growth of Fusarium verticillioides wild-type and LAE1 deletion mutant in liquid fumonisin inducing media (GYAM) Fusarium verticillioides produces a polyketide derived mycotoxin, fumonsin, over time in liquid media, Proctor et al, Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis, Fungal Genetics and Biology, 38:237-249.
Project description:The goal of this RNA-seq experiment was to determine how gene expression in RDEBSCC cell lines was altered relative to normal culture conditions when the culture media was supplemented with TGFb1 or TGFb1 and a TGFb1 inhibitor (SB431542).
Project description:Investigation of whole genome gene expression level changes over time of Fusarium verticillioides wild-type in liquid fumonisin inducing media (GYAM) Fusarium verticillioides produces a polyketide derived mycotoxin, fumonsin, over time in liquid media, Proctor et al, Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis, Fungal Genetics and Biology, 38:237-249 Wild type F. verticillioides strain 7600 cultured for two and five days in liquid GYAM.
Project description:Transcriptional profiling of human preadipocytes comparing preadipocytes cultured in control media vs co-culture with PBMC's after 3 days. Goal was to elucidate novel expression patterns in preadipocytes during exposure to human immune cells. 2-condition experiment, Preadipocytes+Media vs Preadipocytes+PBMC. Biological replicates: 4 experimental replicates.
Project description:Investigation of whole genome gene expression level changes over time of Fusarium verticillioides wild-type in liquid fumonisin inducing media (GYAM). Fusarium verticillioides produces a polyketide derived mycotoxin, fumonsin, over time in liquid media (Proctor et al, Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis, Fungal Genetics and Biology, 38:237-249). A twelve chip study using total RNA recovered from twelve wild type cultures of Fusarium verticillioides. Each chip measures the expression level of 13,288 genes with thirteen 60-mer probe pairs per gene.
Project description:Transcriptome analysis to determine the impact of oral exposure (in a sugar meal) to the liquid supernatant (i.e. LB culture media) of Chromobacterium sp. Panama biofilm culture. The biofilm supernatant (i.e. media) was first filtered with a 0.2uM filter to remove all live bacterial cells. It was then mixed with 10% sucrose, and a control sucrose meal was mixed with filtered LB. Mosquitoes were exposed to each sugar meal for 24 hours and then midguts were dissected from 20 adult females per treatment. The entire experiment was performed 4 independent times.
Project description:Interventions: Experiment Group A:use the ultrasonic surgical systems (Ezisurg Medical Co., Ltd.) in the colorectal surgery;Experiment Group B:use the reusable shear for ultrasonic surgical system (Ezisurg Medical Co., Ltd.) in the colorectal surgery;Control Group:use the Harmonic Ace+7, 5mm Diameter Shears with Advanced Hemostasis (Ethicon Endo-Surgery,LLC) in the colorectal surgery
Primary outcome(s): overall good performance rate
Study Design: Parallel
Project description:Investigation of whole genome gene expression level changes after 6 days growth of Fusarium verticillioides wild-type and LAE1 deletion mutant in liquid fumonisin inducing media (GYAM) Fusarium verticillioides produces a polyketide derived mycotoxin, fumonsin, over time in liquid media, Proctor et al, Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis, Fungal Genetics and Biology, 38:237-249. 6 arrays: Two samples (wild type, LAE1 deletion mutant), one time point (6 days), three biological replicates.