Project description:Cheese samples analyzed using SPME GC/MS. Headspace sampled at 160C with a Polydimethylsiloxane / Divinylbenzene (PDMS/DVB) df 65 um fiber. No prior sample processing or preparation have been carried out.
Project description:This dataset comprises the raw mass spectrometry files generated from the analysis of VOCs extracted from the pulp of Theobroma grandiflorum (Copoazu) at three distinct maturation stages (ripe, overripe, and medium). The study used HS-SPME-GC-MS. A Divinylbenzene/carbon wide-range/polydimethylsiloxane (DVB/CWR/PDMS) 80 um x 10 mm fiber was used in combination with a Gas Chromatograph HP 6890 Series equipped with an Agilent Mass Selective Detector 5973. The VOCs were separated on a capillary GC column of 5% Phenyl/95% Dimethyl Polysiloxane (30 m x 0.25 mm x 0.25 um).
Project description:Volatiles from had space of the cultures of fungus, bacteria and interacting bacteria and fungus. The volatiles are collected from headspace with Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) df 65 um SPME.
Project description:The GC-MS analysis conducted via headspace sampling with a Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) df 65 um Solid Phase Microextraction (SPME) fiber. A Thermo Scientific Trace 1310 GC (TG-5ms 5 30m length, 0.25mm ID, 0.25 �m film thickness column) and a TSQ 8000 EVO mass spectrometer are used. Sputum samples for the WinCF model by RAQ.
Project description:Skin samples collected from underarm w/ PDMS for 30 seconds. Samples used for optimization of GC headspace methodology wrt desorption time, cryofocusing, and size of PDMS patch (10mL vials were used).
Project description:Obtaining multiple sample types from the same exhaled breath condensate (EBC) sample can reduce the number of samples needed for diagnostics purposes, allowing for sampling to be completed quicker and making it even easier to collect breath from patients. In this study, we performed analysis for volatile organic compounds (VOCs) and proteins from the same EBC sample. Pooled EBC samples were split into two groups: three samples that utilized immersion thin film-solid phase microextraction (TF-SPME) sampling for VOCs analysis and three samples that did not undergo TF-SPME sampling (non-TF-SPME). All six EBC samples were analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS) for proteomics analysis. VOCs were analyzed via two-dimensional gas chromatography-mass spectrometry (GC x GC-MS). One hundred and eighty-four VOCs were found to be more abundant in EBC samples compared to blank or controls. There was no significant difference in the number of proteins detected in the TF-SPME samples compared to the non-TF-SPME samples and 144 of the 206 total unique proteins detected were found in both sample groups. These results indicate that TF-SPME sampling does not negatively affect the number of proteins that can be detected in EBC. This work is a step towards linking VOC and protein data together to obtain multi-omics breath data from a single breath sample.
Project description:Samples from extraction of volatile organic compounds from frogs using two sampling methods: in vivo and sampling from the skin and a DVB/CAR/PDMS-SPME fiber. Splitless injection mode using a desorption temperature of 250C. Separation was performed initiating to 40C for 3 min, and then increasing the temperature to 100C using a rate of 6C/min, afterwards incresing to 200C changing the rate to 4C/min, and finally increasing to 300C, using a rate of 20C/min, and maintaing to this temperature for 3 min. Analysis were performed in a GC HP 6890 Series equipped with an Agilent Mass Selective Detector 5973 (Agilent technologies, Palo Alto, CA, USA). Separation was performed on a BP-5 capillary GC column (30 m 0.25 mm 0.25 um, SGE, Austin, TX, USA) using helium as a carrier gas at a flow rate of 1.0 mL/min.
Project description:Skin samples collected from underarm w/ PDMS for 30 seconds. Samples used for optimization of GC headspace methodology wrt desorption time, cryofocusing, and size of PDMS patch (10mL vials were used).
Project description:IL-17 Inhibition in Spondyloarthritis Associates with Subclinical Gut Microbiome Perturbations and a Distinctive IL-25-Driven Intestinal Inflammation
Volatile short-chain fatty acids analysis.
Approximately 50 mg of sample were placed in 2 ml borosilicate vials capped with a screw cap with silicone septum. The capped vials were stored at -20 C and thawed immediately prior to analysis. The GC-MS analysis was carried out using the Agilent 7200 GC/QTOF equipped with robotic sampler system. The volatiles from the sample were extracted from headspace using Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) df 65 um Solid Phase Microextraction Fiber (SPME) for 30 minutes at 50 C. The fiber was then inserted into the injector equipped with Merlin septum heated to 250 C and the adsorbed compounds were desorbed for 1 minute.