Project description:Cheese samples analyzed using SPME GC/MS. Headspace sampled at 160C with a Polydimethylsiloxane / Divinylbenzene (PDMS/DVB) df 65 um fiber. No prior sample processing or preparation have been carried out.
Project description:This dataset comprises the raw mass spectrometry files generated from the analysis of VOCs extracted from the pulp of Theobroma grandiflorum (Copoazu) at three distinct maturation stages (ripe, overripe, and medium). The study used HS-SPME-GC-MS. A Divinylbenzene/carbon wide-range/polydimethylsiloxane (DVB/CWR/PDMS) 80 um x 10 mm fiber was used in combination with a Gas Chromatograph HP 6890 Series equipped with an Agilent Mass Selective Detector 5973. The VOCs were separated on a capillary GC column of 5% Phenyl/95% Dimethyl Polysiloxane (30 m x 0.25 mm x 0.25 um).
Project description:Volatiles from had space of the cultures of fungus, bacteria and interacting bacteria and fungus. The volatiles are collected from headspace with Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) df 65 um SPME.
Project description:Skin samples collected from underarm w/ PDMS for 30 seconds. Samples used for optimization of GC headspace methodology wrt desorption time, cryofocusing, and size of PDMS patch (10mL vials were used).
Project description:The GC-MS analysis conducted via headspace sampling with a Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) df 65 um Solid Phase Microextraction (SPME) fiber. A Thermo Scientific Trace 1310 GC (TG-5ms 5 30m length, 0.25mm ID, 0.25 �m film thickness column) and a TSQ 8000 EVO mass spectrometer are used. Sputum samples for the WinCF model by RAQ.
Project description:Obtaining multiple sample types from the same exhaled breath condensate (EBC) sample can reduce the number of samples needed for diagnostics purposes, allowing for sampling to be completed quicker and making it even easier to collect breath from patients. In this study, we performed analysis for volatile organic compounds (VOCs) and proteins from the same EBC sample. Pooled EBC samples were split into two groups: three samples that utilized immersion thin film-solid phase microextraction (TF-SPME) sampling for VOCs analysis and three samples that did not undergo TF-SPME sampling (non-TF-SPME). All six EBC samples were analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS) for proteomics analysis. VOCs were analyzed via two-dimensional gas chromatography-mass spectrometry (GC x GC-MS). One hundred and eighty-four VOCs were found to be more abundant in EBC samples compared to blank or controls. There was no significant difference in the number of proteins detected in the TF-SPME samples compared to the non-TF-SPME samples and 144 of the 206 total unique proteins detected were found in both sample groups. These results indicate that TF-SPME sampling does not negatively affect the number of proteins that can be detected in EBC. This work is a step towards linking VOC and protein data together to obtain multi-omics breath data from a single breath sample.
Project description:Samples from extraction of volatile organic compounds from frogs using two sampling methods: in vivo and sampling from the skin and a DVB/CAR/PDMS-SPME fiber. Splitless injection mode using a desorption temperature of 250C. Separation was performed initiating to 40C for 3 min, and then increasing the temperature to 100C using a rate of 6C/min, afterwards incresing to 200C changing the rate to 4C/min, and finally increasing to 300C, using a rate of 20C/min, and maintaing to this temperature for 3 min. Analysis were performed in a GC HP 6890 Series equipped with an Agilent Mass Selective Detector 5973 (Agilent technologies, Palo Alto, CA, USA). Separation was performed on a BP-5 capillary GC column (30 m 0.25 mm 0.25 um, SGE, Austin, TX, USA) using helium as a carrier gas at a flow rate of 1.0 mL/min.
Project description:Chimonocalamus is the only bamboo species known to secrete aromatic oils. The species is named after the distinctive aromatic oil found within the cavity of its bamboo stem. This study employed sensory analysis, headspace-gas chromatography-mass spectrometry (HS-SPME-GC-MS), and transcriptome sequencing to investigate the volatile component composition and formation mechanisms in Chimonocalamus delicatus, an endemic bamboo species of Yunnan Province. The bamboo samples analyzed included stems at different stem ages, as well as various parts. The aroma components of Chimonocalamus delicatus primarily consist of sesquiterpenes, including Humulene and Caryophyllene, which are terpenoid compounds. Principal component analysis (PCA) revealed significant differences in the volatile compounds across various stem ages and plant parts. This may constitute a key source of the characteristic aroma of Chimonocalamus delicatus at different stem ages and plant parts. Transcriptome analysis revealed that the aroma of Chimonocalamus delicatus was predominantly enriched in the secondary metabolite pathways, including the MVA and MEP pathways. The enzyme activities of key enzymes in its metabolic pathway were also determined. A comprehensive analysis of differential aroma metabolites and differentially expressed genes (DEGs) in Chimonocalamus delicatus led to the identification of key genes (DXR, FPS, TPS6, TPS7) involved in the regulation of aroma compound biosynthesis. In this study, the volatile components and regulatory network of Chimonocalamus delicatus essential oil were analyzed, which provided a solid theoretical basis for the development of bioactive compounds in Chimonocalamus delicatus.
Project description:Skin samples collected from underarm w/ PDMS for 30 seconds. Samples used for optimization of GC headspace methodology wrt desorption time, cryofocusing, and size of PDMS patch (10mL vials were used).