GNPS-UTEP_Chagas disease vaccine_Baboon heart metabolomics_June 2017
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ABSTRACT: Baboons were immunized with L-MPLA adjuvant only, or adjuvant+KM24h, +MASPpep, or +KM24h&MASPpep combination. Animals were challenged twice with Trypanosoma cruzi. Hearts were collected 168 days post-infection. Ventricles were divided and sectioned. Metabolites were extracted by a two-step protocol, first with 50% methanol (aqueous extract) then with 3:1 dichloromethane:methanol (organic extract). Liquid chromatography separation was performed on a Kinetex C8 LC Column 50 x 2.1 mm, with water+0.1% formic acid and acetonitrile+0.1% formic acid as mobile phases.
INSTRUMENT(S): Q Exactive Plus
ORGANISM(S): Papio Hamadryas (ncbitaxon:9557) Trypanosoma Cruzi (ncbitaxon:5693)
SUBMITTER:
Laura-Isobel McCall
Igor C. Almeida
PROVIDER: MSV000081160 | GNPS | Wed Jun 14 15:00:00 BST 2017
REPOSITORIES: GNPS
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