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GNPS - Ecoli_strains_extracellular_metabolome_comparisons_Study1

ABSTRACT: Data from a comparative metabolomics study of extracellular metabolomes of 10 E coli strains of phylogroup B2 (5 strains; UPEC 536, APEC O1, CFT073, J96, Nissle 1917) and other phylogroups (5 strains; 042, MG1655, HS, O103:H2, TUV93-0). pHILIC-MS full scan data of all supernatant extracts (three replicates - 1:3:1 H20-methanol-chloroform extractions) was obtained in alternating ionization mode: MzXML files with positive ionization mode spectra and negative ionization mode spectra are provided. Controls (blanks, solvent and medium controls, and pooled samples run across the batch, are included as well). pHILIC-MS/MS data of selected supernatant as well as whole cell extract samples was obtained for metabolite annotation purposes. The resulting MzXML files in positive ionization mode and negative ionization mode are provided.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli (ncbitaxon:562)

SUBMITTER: Justin van der Hooft

PROVIDER: MSV000081287 | GNPS | Wed Jul 12 05:00:00 BST 2017

REPOSITORIES: GNPS

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Project description:Purpose: In this work, we evaluated the role of two indicative species, Citrobacter werkmanii (CW) and Escherichia albertii (EA), in the virulence of two DEC pathotypes, Shiga toxin-producing (STEC) and enteroaggregative (EAEC) Escherichia coli. Methods: To determine the effect of supernatant obtained from CW and EA cultures in STEC strain 86-24 and EAEC strain 042 gene expression, a RNA-seq analysis was performed. T84 cells were infected with DEC strains in the presence or absence of supernatant from EA and IL-8 secretion was evaluated. The effect of supernatant from EA on the growth and adherence of STEC and EAEC to T84 cells was also evaluated. Finally, we studied the participation of long polar fimbriae (Lpf) in STEC and plasmid-encoded toxin (Pet) in EAEC during DEC infection in the presence of supernatant from EA. Results: RNA-seq analysis revealed that several virulence factors in STEC and EAEC were up-regulated in the presence of supernatants from CW and EA. Interestingly, an increase in the secretion of IL-8 was observed in T84 cells infected with STEC or EAEC in the presence of a supernatant from EA. Similar results were observed with the supernatants obtained from clinical strains of E. albertii. Supernatant from EA had no effect on the growth of STEC and EAEC, or on the ability of these DEC strains to adhere to intestinal epithelial cells. Finally, we found that Pet toxin in EAEC was up-regulated in the presence of a supernatant from EA. In STEC, using mutant strains for Lpf fimbriae, our data suggested that these fimbriae might be participating in the increase of IL-8 induced by STEC on intestinal epithelial cells in the presence of a supernatant from EA. Conclusion:Supernatant obtained from an indicative species of DEC-positive diarrhea could modulate gene expression in STEC and EAEC, and IL-8 secretion induced by these bacteria. These data provide new insights into the effect of gut microbiota species in the pathogenicity of STEC and EAEC.

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GNPS - Ecoli_strains_extracellular_metabolome_comparisons_Study1

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