Project description:Fungi are ubiquitous and are often confronted with the need to secure utilisable carbon from their external growth milieu through the use of extracellular proteins to scavenge for carbon from a vast array of complex polymeric carbon sources. This attribute is conserved across evolution in fungi. To understand how filamentous fungi extracellular proteins are modulated in response to the presence polymeric carbons in the environment, we have typed the array of the main extracellular proteins involved and their dynamics using a known hypercellulolytic fungus – Penicillium funiculosum (NCIM 1228), through multiplexed quantitative proteomics
Project description:Chemical investigations of Penicillium sp. CMB-STF067 is based on both the antibacterial property of its extract and the Global Natural Product Social (GNPS) molecular networking analysis of 176 soil-associated fungi, guided the isolation of 4-new xanthoquinodins, jugiones A-D.
Project description:Cudrania tricuspidata extracts and 3 major compounds, including 4'-O-Methylalpinumisoflavone, Alpinumisoflavone and 6,8-diprenylgenistein
Project description:Chronic Inflammation has a key role in the development of insulin resistance and type 2 diabetes. Previously, we demonstrated that OBE100, a natural extract from the leaves of Eucalyptus tereticornis, has anti-inflammatory properties. Three pentacyclic triterpenoids, ursolic acid, oleanolic acid, and ursolic acid lactone are the major compounds present in OBE100. These molecules have shown multiple biological activities. In this study we analyzed how the compounds of OBE100 modify macrophage gene expression using RNA sequencing. Triterpenoids regulate the inflammatory program in activated macrophages inhibiting the expression of many cytokines, chemokines, and inflammatory mediators. However, the OBE100 extract has a more powerful immunomodulatory effect than the triterpene mixture increasing the number of genes regulated, both in mouse and human models. Our study shows that OBE100 is a promising extract for the treatment of diabetes that can break the link between inflammation and insulin resistance.
Project description:We compared the gene expression stimulated with fungal extracts from Aspergillus (A.) fumigatus, Alternaria (A.) alternata, or Penicillium (P.) notatum in NCI-H292 (a human bronchial epithelial cell line) to search Allergic bronchopulmonary mycosis (ABPM)-related genes. We identified a mucin-related MUC5AC gene, the expression of which was selectively induced by A. fumigatus. Total RNA from NCI-H292 cells stimulated for 24 h with the A. fumigatus, A. alternata, or P. notatum fungi extracts was extracted and subjected to microarray analysis. Each experiments were perfomed once for each stimulus.
Project description:MS2 collected on qTOF, UPLC C18 gradient of 10-100% ACN over first 12 minutes, held at 100% ACN for minute 12-14. Fungi and Bacteria grown alone and pairwise on Cheese Curd Agar, naming is fungi first then bacterial partner (if it is a pairwise culture), then biological replicate number.
CCA = cheese curd agar media control
Ecoli = E. coli K12
JB418 = Pseudomonas psychrophila sp. JB418
12 = Penicillium solitum
SAM = Penicillium camemberti
RS17 = Penicillium atramentosum
Fus = Fusarium domesticum
JB370 = Scopulariopsis strain JB370
1655 = Scopulariopsis strain 165-5
Can = Candida catenulata
Deb = Debaryomyces hansenii