Project description:Comparison between miRNA expression in plasma of women with and without metabolic syndrome. We used microarrays to compare the composition of miRNAs in plasma of participants with and without metabolic syndrome (ATP III criteria).
Project description:Metabolic syndrome is a common and complicated metabolic disorder and defined as a clustering of metabolic risk factors such as insulin resistance or diabetes, obesity, hypertension, and hyperlipidemia. The identification of accurate and effective biomarkers is beneficial to the early diagnosis and treatment of metabolic syndrome. Our study firstly detected the plasma miRNA expression profile of MetS patients compared with control group by high-throughput sequencing and integrated bioinformatics approaches. To our best knowledge, our study firstly perform high-throughput sequencing to obtain the circulating microRNA expression data in MetS plasma, and identified several potential plasma biomarkers for MetS.
Project description:In this work, plasma samples of 5 metabolic syndrome patients and 5 healthy volunteers were collected. Then, high-throughput RNA sequencing was performed to detect the expression of plasma coding RNA.
Project description:Interventions: CRA vs Control:No
Primary outcome(s): Rate of metabolic syndrome;adipokines;visceral fat;Values of metabolic syndrome elements
Study Design: Case-Control study
Project description:Abstract The metabolic syndrome is a cluster of conditions that predispose for diabetes and cardiovascular disease. Nine metabolic syndrome patients were recruited to 48 workouts of interval training. At the end of the study, all patients significantly reduced their risk of cardiovascular disease (in terms of VO2max, blood pressure and plasma lipid). Exercise-induced transcriptional changes may provide new mechanistically insights in the area of improved health by exercise. Aim: Determine whether transcriptional changes occurred in the blood cells of patients after the exercise program. We hypothesized that significantly altered biological processes in blood would include a set of genes at least partly responsible for the improvement seen in this patient group after the exercise programme. Methods: Blood from five patients were collected in PAXgene tubes pre and post the exercise period. RNA was extracted and run on microarrays. Results: Gene set enrichment analysis revealed twelve significantly enriched biological processes and molecular functions that were up-regulated post exercise. Seven processes and functions were down-regulated (nom p-value <0.05). Exercise induced a down-regulation of plasma mRNA- and protein levels of arginase-I and vWf, which might explain improved risk profile and endothelial function of the metabolic syndrome patients. Conclusion: After the exercise period was completed, the metabolic syndrome patients had decreased transcription of genes associated with blood clotting and steroid metabolism. Arginase-I was decreased post exercise, which may explain the previous reported improved NO-availability and endothelial function. Keywords: aerobic capacity, cardiovascular disease, microarray, arginase, blood clotting
Project description:The goals of this study are to investigate the molecular mechanism and biomarker by DNA methylation profiles in patients with metabolic syndrome compared to normal.
Project description:The goals of this study are to investigate the molecular mechanism and biomarker by DNA methylation profiles in patients with metabolic syndrome compared to normal.