Project description:<p>The Unfolded Protein Response (UPR) is a retrograde, ER-to-nucleus, signalling pathway which is conserved across kingdoms. In plants, it contributes to development, reproduction, immunity and tolerance to abiotic stress. This lipidomic dataset was produced from Arabidopsis thaliana mature seeds. Wild-type (WT) and mutant deficient for UPR actors (bzip60, ire1a and ire1b single mutants, as well as double ire1 mutants) were used as genetic backgrounds to investigate whether UPR controls lipid seed filling in Arabidopsis thaliana. Our experimental setup also allows to distinguish among differentially-overaccumulated lipids which ones are dependent on IRE1s and/or bZIP60.</p>
Project description:<p>The Unfolded Protein Response (UPR) is a retrograde, ER-to-nucleus, signalling pathway which is conserved across kingdoms. In plants, it contributes to development, reproduction, immunity and tolerance to abiotic stress. This metabolomic dataset was produced from 14-day-old Arabidopsis thaliana seedlings challenged by tunicamycin (Tm), an antibiotic inhibiting Asn-linked glycosylation in the endoplasmic reticulum (ER), causing an ER stress and eventually activating the UPR. Wild-type (WT), double mutant deficient for two main actors of the UPR (INOSITOL-REQUIRING ENZYME 1A [IRE1A] and INOSITOL-REQUIRING ENZYME 1B [IRE1B]), as well as doubles mutants complemented with either of the WT IRE1 alleles were used as genetic backgrounds in our experimental setup. This allows to distinguish among differentially-overaccumulated metabolites which ones are dependent on IRE1A and/or IRE1B. Also, shoots and roots were harvested separately to determine organ-specific metabolic responses to Tm.</p>
Project description:<p>The Unfolded Protein Response (UPR) is a retrograde, ER-to-nucleus, signalling pathway which is conserved across kingdoms. In plants, it contributes to development, reproduction, immunity and tolerance to abiotic stress. This metabolomic dataset was produced from 14-day-old Arabidopsis thaliana seedlings challenged by tunicamycin (Tm), an antibiotic inhibiting Asn-linked glycosylation in the endoplasmic reticulum (ER), causing an ER stress and eventually activating the UPR. Wild-type (WT), bzip60 single mutant and the double mutant deficient for two main actors of the UPR (INOSITOL-REQUIRING ENZYME 1A and INOSITOL-REQUIRING ENZYME 1B) were used as genetic backgrounds in our experimental setup, allowing to distinguish among differentially-overaccumulated metabolites which ones are dependent on IRE1s and/or bZIP60. Also, shoots and roots were harvested separately to determine organ-specific metabolic responses to Tm.</p>
Project description:A thaliana WT and ST2a mutant dataset with time points 0-6h post wounding. Data was generated on a Thermo Q Exactive and C18 RP UPLC. Positive polarity acquisition on LC-MS/MS
Project description:A thaliana WT and ST2a mutant dataset with time points 0-6h post wounding. Data was generated on a Thermo Q Exactive and C18 RP UPLC. Negative polarity acquisition on LC-MS/MS
Project description:This dataset presents the results of a label-free quantitative proteomic analysis of seed mucilage (from 10 DPA siliques) of Arabidopsis thaliana wild-type (WT) and upl3 mutant plants. UPL3 encodes a HECT-type E3 ubiquitin ligase; by identifying differentially expressed proteins between WT and upl3, we focused on proteins related to cell wall polysaccharides, aiming to investigate the molecular mechanism by which UPL3 regulates seed mucilage structure. The dataset includes raw mass spectrometry data, protein identification results, and quantitative information of differential proteins, which can provide data support for research on the regulation of plant cell wall biosynthesis.