Project description:Combining Mass Spectrometric Metabolic Profiling with Genomic Analysis: A Powerful Approach for Discovering New Natural Products from Cyanobacteria
Karin Kleigrewe, Jehad Almaliti, Isaac Yuheng Tian, Robin B. Kinnel, Anton Korobeynikov, Emily A. Monroe, Brendan M. Duggan, Vincenzo Di Marzo, David H. Sherman, Pieter Dorrestein, Lena Gerwick and William H. Gerwick
Project description:Small RNA sequencing of sorted cell type, mice kidneys, after unilateral ureteral obstructive (UUO) surgery after 2 days, 7 days and a reversible (rUUO) 14 day model with corresponding sham operated mice. The goal of the study is to identify micro-RNA dysregulation of expression between different cell types corresponding with kidney injury, in order to identify and assess biomarkers which could be translated into the clinical setting.
Project description:Cyanobacteria extracts and SPE fractions. LC MSMS analysis was performed in an UltiMate 3000 UPLC system (Thermo Scientific) using a Kinetex 1.7 mm C18 reversed phase UHPLC column (50 X 2.1 mm) and Maxis Impact HD Q-TOF mass spectrometer (Bruker Daltonics) equipped with ESI source.
Project description:RNA sequencing of sorted cell types, isolated from mice kidneys, after unilateral ureteral obstructive (UUO) surgery after 2 days, 7 days and a reversible (rUUO) 14 day model with corresponding sham operated mice. The goal of the study was to identify temporal gene expression changes in different cell types corresponding with kidney injury, in order to understand cell specific transcriptomic changes in the context of kidney injury and repair.