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GNPS - In situ temporal changes in VOCS emission of Dendrobates auratus using PDMS films for in vivo sampling

ABSTRACT: Samples from extraction of volatile organic compounds from frogs using PDMS films and posterior thermal desorption using an incubation time of 20 min to 190C. Splitless injection using a syringe to 145C. Inhlet temperature of 250C. Separation was performed initiating to 40C for 3 min, and then increasing the temperature to 100C using a rate of 6C/min, afterwards incresing to 200C changing the rate to 4C/min, and finally increasing to 300C, using a rate of 20C/min, and maintaing to this temperature for 3 min. Analysis were performed in a Agilent 7200 GC-MS QTof (Agilent technologies, Palo Alto, CA, USA). Separation was performed HP-5MS capillary GC column (30 m 0.25 mm 0.25 um, Agilent technologies, Palo Alto, CA, USA) using helium as a carrier gas at a flow rate of 1.0 mL/min.

INSTRUMENT(S): Agilent 7200 GC-MS QTof

ORGANISM(S): Dendrobates Auratus (ncbitaxon:43471)

SUBMITTER: Mabel Gonzalez

PROVIDER: MSV000084856 | GNPS | Wed Jan 29 16:48:00 GMT 2020

REPOSITORIES: GNPS

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