ABSTRACT: Third flash with F1 fraction obtained by RG on the 15/05/2018. This flash contains tube number 45 generated during flash 2 (ispropanol accidental injection).
Project description:Sub-fractions obtained from F1 fraction obtained on the 15/05/2018 by RG. These sub-fractions were diluted in EtOAc and then MeOH (for UPLC-MS analysis).
Project description:For RNA-seq experiments, 20 embryos were placed into 5 mL final volume of sterile ERM. Each treatment group were exposed within a 10-minute window. (N=5 beakers per treatment). On 7 days post fertilization (dpf), fish were transferred to a labeled and pre-weighed 1.7 mL tube. The tube was then immediately flash frozen in liquid nitrogen prior to RNA-seq analysis.
Project description:For RNA-seq experiments, 20 embryos were placed into 5 mL final volume of sterile ERM. Each treatment group were exposed within a 10-minute window. Exposure groups included ERM, [100 ng/L AVS or 1 µg/L AVS] or [100 ng/L or 1 µg/L afidopyropen] (N=5 beakers per treatment). On 7 days post fertilization (dpf), fish were transferred to a labeled and pre-weighed 1.7 mL tube. The tube was then immediately flash frozen in liquid nitrogen prior to RNA-seq analysis.
Project description:To obtain new insights for heterosis mechanisms in Arabidopsis, transcript profiling of arabidopsis F1 hybrid and their parentral lines was obtained from Affymetrix GeneChips ATH array. The microarray analysis exhibited that there were 44 up-regulated and 12 down-regulated genes with more than a 1.5-fold changes in the profiles of F1 hybrid against those of each parent. Gene ontology analyses highlighted up-regulated genes as organic nitrogen-related in the F1 hybrid.
Project description:First generation animals (F1) were exposed to multiple courses of synthetic glucocorticoids in utero. Animals were then bred to produce second (F2) and third (F3) generation offspring. These animals underwent behavioural assessmenets before sacrifice. In this experiment the prefrontal cortex for F1, F2, and F3 female animals, as well as F1 males (Control and sGC) have been extracted via micro-punch and RNA has been extracted.
Project description:First generation animals (F1) were exposed to multiple courses of synthetic glucocorticoids in utero. Animals were then bred to produce second (F2) and third (F3) generation offspring. These animals underwent behavioural assessmenets before sacrifice. In this experiment the prefrontal cortex for F1, F2, and F3 female animals, as well as F1 males (Beta and Control) have been extracted via micro-punch and RNA has been extracted.
Project description:For RNA-seq experiments, 20 embryos were placed into 5 mL final volume of sterile ERM. Each treatment group were exposed within a 10-minute window. Exposure groups included ERM, 1 µg/L, and 100 µg/L ifosfamide or 10 and 1000 µg/L citalopram (N=5 beakers per treatment). On 7 days post fertilization (dpf), fish were transferred to a labeled and pre-weighed 1.7 mL tube using a p1000 with the tip cut off, then water was completely removed using a p200. The tube was then immediately flash frozen in liquid nitrogen prior to RNA-seq analysis.