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GNPS_GC_Derivatized human blood serum spiked with standards of FAMEs and hydrocarbons

ABSTRACT: Post-thawing, human serum samples (30 uL) were sequentially extracted using solvent extraction, once with 1 mL of acetonitrile: isopropanol: water (3:3:2, v/v) ratio , followed by another extraction with 500 uL of acetonitrile: water (1:1, v/v) ratio mixture at 4 C. Adonitol (Cat. No. A5502-5G, Sigma-Aldrich; 5 uL from a 10 mg/ml stock), an internal standard was added to all samples prior to solvent extraction. The pooled extracts (~ 1500 uL) resulting from the two steps were dried in vacuo at 4 C prior to chemical derivatization. Dummy extractions were performed in blank tubes that served as extraction blanks for background subtraction of noise resulting from extraction solvents, derivatization reagents, unwanted sources, and other sources of contamination from the analytical platform, i.e., septa, liner, column, vials, handling etc. All samples (i.e., except FAMEs and n-alkanes) were sequentially derivatized with methoxyamine hydrochloride (MeOX) and 1% TMCS in N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA).

INSTRUMENT(S): QExactive-GC

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Biswapriya Misra

PROVIDER: MSV000085128 | GNPS | Fri Mar 20 12:30:00 GMT 2020

REPOSITORIES: GNPS

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Project description:This a model from the article: Critical study of and improvements in chromatographic methods for the analysis of type B trichothecenes. Mateo JJ, Llorens A, Mateo R, Jimenez M. J Chromatogr A 2001 May 18;918(1):99-112 11403460 , Abstract: Various analytical methods used in the analysis of type B trichothecenes (deoxynivalenol, nivalenol, 3- and 15-acetyldeoxynivalenol) in cereals were compared and optimised in this work. These methods use either GC-electron-capture detection (ECD) of trimethylsilyl, trifluoroacetyl and heptafluorobutyryl derivatives or HPLC with UV or photodiode array detection of analytes. A new HPLC procedure using fluorescence detection prior derivatisation with coumarin-3-carbonyl chloride has been also tested. Five extraction solvents and two solid-phase extraction cartridges (silica, Florisil) plus a especial clean-up column (MycoSep 225) were compared in order to obtain the best recovery of the mycotoxins with minimal presence of coextractives in the chromatograms. The chosen extraction solvent was a mixture of acetonitrile-water (84:16, v/v). The MycoSep 225 column was chosen as the best alternative for clean-up of grain samples. For GC-ECD analysis, derivatisation of analytes with heptafluorobutyric anhydride prior the final determination was chosen as the most suitable procedure. HPLC-photodiode array (at 221 nm) analysis was more suitable for determination of type B trichothecenes than HPLC of the fluorescent coumarin-3-carbonyl derivatives. Recoveries obtained in spiked corn, rice and wheat are reported. The utility of the proposed methodology was assayed in cereal cultures of various Fusarium strains. This model was taken from the CellML repository and automatically converted to SBML. The original model was: Mateo JJ, Llorens A, Mateo R, Jimenez M. (2001) - version=1.0 The original CellML model was created by: Catherine Lloyd c.lloyd@auckland.ac.nz The University of Auckland This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not.. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.

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GNPS_GC_Derivatized human blood serum spiked with standards of FAMEs and hydrocarbons

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