ABSTRACT: Crude extracts and fractions of the liquido culture of bacteria Actinomadura sp. BRA177 recovered from Saint Peter and Saint Paul Archipelago
Project description:Data acquired by LC-MS in full scan mode (MS1) in a five point dilution series of methanolic extracts (methanol:water) for actinomycetes BRA006 (Micromonospora sp.), BRA010 (Streptomyces sp.) and BRA177 (Actinomadura sp.).
Project description:We addressed the optimization of the xylanolytic activity in extracellular enzymatic extracts of Cellulomonas sp. B6 and C. fimi B-402 for their subsequent application in lignocellulosic biomass hydrolysis, by culture in several substrates. As demonstrated by secretomic profiling, wheat bran and waste paper resulted to be suitable inducers for the secretion of xylanases of Cellulomonas sp. B6 and C. fimi B-402, respectively. Both strains showed high xylanolytic activity in culture supernatant although Cellulomonas sp. B6 was the most efficient xylanolytic strain.
Project description:MS/MS data obtained from crude extracts from Pseudoalteromonas sp. This data is not publisher yet, so, contact Mr. Christian Martin before use it.
Project description:Data obtained by LC-MS/MS of methanolic extracts (methanol:water) for the actinomycetes BRA006 (Micromonospora sp.), BRA010 (Streptomyces sp.) and BRA177 (Actinomadura sp.), where the precursor ions were guided by a scheduled precursor list (SPL).
Project description:Targeted therapies against cancer stem cells, which are enriched in side populations (SP), involves interruption of Wnt-signalling. Furthermore, EpCAM is a SP marker and modulator of Wnt-signalling. Therefore, the effects of an anti-EpCAM treatment on SP-cells and WNT/β-catenin signalling was studied. SP of the human lung adenocarcinoma cell line A549 was obtained by fluorescence activated cell sorting and whole genome scans helped to define their molecular phenotype after anti-EpCAM antibody treatment. Anti-EpCAM treated and untreated A549 cells were subjected to Hoechst 33342 dye exclusion assay and sorted to SP and non-SP fractions by FACS. Three biological replicates.
Project description:Targeted therapies against cancer stem cells which are enriched in side populations (SP) involves interruption of Wnt-signalling. Furthermore, EpCAM is a SP marker and modulator of Wnt-signalling. Therefore, the effects of an anti-EpCAM treatment on SP-cells and WNT/M-NM-2-catenin signalling was studied. SP of the murine lung adenocarcinoma cell line A2C12 was obtained by fluorescence activated cell sorting and whole genome scans helped to define their molecular phenotype after anti-EpCAM antibody treatment. Anti-EpCAM treated and untreated A2C12 cells were subjected to Hoechst 33342 dye exclusion assay and sorted to SP and non-SP fractions by FACS. Three biological replicates.
Project description:LC-MS/MS data of crude extracts produced by isolated bacteria recovered from Brazilian Rocas Atoll. Subset of MSV000083601 for use as an example dataset on the Workshop on Advanced Mass Spectometry 2025.
Project description:Investigation of whole genome gene expression level in motile strain of Sphingomonas. sp A1 All flagellar genes in motile strain of Sphingomonas. sp A1 are highly transcribed. A two chip study using total RNA recovered from wild-type and motile strains of Sphingomonas. sp A1 grown in 0.5% alginate medium.