Project description:While our previous evolutionary analyses of Salinispora and its BGCs have focused on 118 strains isolated from worldwide marine sediment samples, the aim of this study was to isolate, genome-sequence, and characterize the biosynthetic potential of a "microscale" collection of Salinispora strains isolated from a 1-m^2 quadrat in Fiji, thus allowing us to compare taxonomic and BGC diversity at different spatial scales. This MassIVE data set contains LCMS runs of the ethyl acetate extracts produced from liquid cultures of 99 microscale Salinispora strains (96 are classified as S. arenicola, 2 as S. pacifica, and 1 as S. oceanensis) grown in A1 media, along with two media controls. The extracts were analyzed on a Agilent 1260 Infinity Series LC system coupled to a 6530 Series Q-TOF mass spectrometer, using a C18 Phenomenex Luna column (5 um, 100 mm x 4.6 mm) with a 10 min solvent gradient from 10% to 100% MeCN (0.1% FA) in water (0.1% FA) with 1.0 mL min-1 flow rate. LCMS parameters: positive mode, source ionization 0 eV, CID 30 eV and a 100-1700 Da detection window. Raw .d and converted .mzxml file formats are included.
Project description:This submission contains the proteomic data from the paper “Culinary diversity in early modern Brazil revealed by residues analysis of ceramic artefacts” by Di Muro et al.. In this paper the culinary use of ceramic during the late 19th and early 20th century in Brazil is investigated through biomolecular analysis of organic residues preserved in the ceramic matrix, including palaeoproteomics. Protein recovery from the ceramic vessels was extremely limited, which is in line with expected protein degradation due to the warm and humid climate, as well as cooking-induced protein degradation. Nevertheless, ancient fish (Sciaenidae) and chicken proteins could be recovered from historical ceramics. The broad taxonomic identification and limited protein recovery highlight both the potential of applying palaeoproteomics to ceramics from tropic climates, as well as the challenges.
Project description:Metabolomics (LC-MS/MS) of propolis and geopropolis samples produced by different species of stingless bees, collected in the city of Bandeirantes (Parana, Brazil). Commercial samples of green and red propolis from Appis mellifera honeybee were also analyzed.