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GNPS - Human NVU - Fetal Brain Pericytes -Methamphetamine

ABSTRACT: For human brain pericytes, we used fetal cells passage 3 (ScienCell, San Diego, CA, USA ) seeded Pericyte medium (ScienCell, San Diego, CA, USA) at density 8-15000 cells/cm2 tissue culture plastic culture dishes coated with Attachment Factor for 30 min (Cell Systems, Kirkland, WA, USA). Methamphetamine (Sigma-Aldrich, St Louis, MO, USA) was prepared at a stock concentration of 10mM in distilled water. Methamphetamine dilutions 50 micromolar were made in Endo and Neuro/Astro/Peri media respectively and applied to the cells for 26 hrs. Further info in "Proteomic and metabolomic characterization of human neurovascular unit cells in response to methamphetamine" Herland et al Adv Biosystems 2020

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anna Herland

PROVIDER: MSV000085820 | GNPS | Thu Jul 23 13:29:00 BST 2020

REPOSITORIES: GNPS

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