GNPS - Anaerobic_fungi_are_an_untapped_reservoir_of_natural_products
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Caecomyces Churrovis (ncbitaxon:2019372) Piromyces Finnis (ncbitaxon:1754191) Neocallimastix Californiae (ncbitaxon:1754190) Anaeromyces Robustus (ncbitaxon:1754192)
SUBMITTER: 
Michelle O'Malley
PROVIDER: MSV000085907 | GNPS | Tue Aug 04 13:35:00 BST 2020
REPOSITORIES: GNPS
Similar Datasets
Project description:Anaerobic fungi (class Neocallimastigomycetes) thrive as low-abundance members of the herbivore digestive tract. The genomes of anaerobic gut fungi are poorly characterized and have not been extensively mined for the biosynthetic enzymes of natural products such as antibiotics. Here, we investigate the potential of anaerobic gut fungi to synthesize natural products that could regulate membership within the gut microbiome. Complementary 'omics' approaches were combined to catalog the natural products of anaerobic gut fungi from four different representative species: Anaeromyces robustus (A. robustus), Caecomyces churrovis (C. churrovis), Neocallimastix californiae (N. californiae), and Piromyces finnis (P. finnis). In total, 146 genes were identified that encode biosynthetic enzymes for diverse types of natural products, including nonribosomal peptide synthetases and polyketide synthases. In addition, N. californiae and C. churrovis genomes encoded seven putative bacteriocins, a class of antimicrobial peptides typically produced by bacteria. During standard laboratory growth on plant biomass or soluble substrates, 26% of total core biosynthetic genes in all four strains were transcribed. Across all four fungal strains, 30% of total biosynthetic gene products were detected via proteomics when grown on cellobiose. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) characterization of fungal supernatants detected 72 likely natural products from A. robustus alone. A compound produced by all four strains of anaerobic fungi was putatively identified as the polyketide-related styrylpyrone baumin. Molecular networking quantified similarities between tandem mass spectrometry (MS/MS) spectra among these fungi, enabling three groups of natural products to be identified that are unique to anaerobic fungi. Overall, these results support the finding that anaerobic gut fungi synthesize natural products, which could be harnessed as a source of antimicrobials, therapeutics, and other bioactive compounds. See publication: https://www.doi.org/10.1073/pnas.2019855118.
This research was performed under the Facilities Integrating Collaborations for User Science (FICUS) program (proposal:https://doi.org/10.46936/fics.proj.2018.50386/60000039) and used resources at the DOE Joint Genome Institute (https://ror.org/04xm1d337) and the Environmental Molecular Sciences Laboratory (https://ror.org/04rc0xn13), which are DOE Office of Science User Facilities operated under Contract Nos. DE-AC02-05CH11231 (JGI) and DE-AC05-76RL01830 (EMSL).
2024-02-14 | MSV000094092 | MassIVE
Project description:Dichloromethane (DCM) extracts of aerial parts and roots of Waltheria indica analyzed in UHPLC-MS/MS in positive ionization mode. .raw, .mzML and MzMine2 processed files(spectra .mgf and feature table .csv) are available.
References:
Cretton, Sylvian, Stéphane Dorsaz, Antonio Azzollini, Quentin Favre-Godal, Laurence Marcourt, Samad Nejad Ebrahimi, Francine Voinesco, et al. 2016. “Antifungal Quinoline Alkaloids from Waltheria Indica.” Journal of Natural Products 79 (2): 300–307.
Cretton, S., L. Breant, L. Pourrez, C. Ambuehl, L. Marcourt, S. N. Ebrahimi, M. Hamburger, et al. 2014. “Antitrypanosomal Quinoline Alkaloids from the Roots of Waltheria Indica.” Journal of Natural Products 77 (10): 2304–11.
2021-12-06 | MSV000088521 | MassIVE
Project description:454 dataset for Hosia, et al. Torstensson et al. Dinasquet et al.
| PRJEB7907 | ENA
Project description:The recent release of a large number of genomes from ectomycorrhizal, orchid mycorrhizal and root endophytic fungi have provided deep insight into fungal lifestyle-associated genomic adaptation. Comparative analyses of symbiotic fungal taxa showed that similar outcomes of interactions in distant related root symbioses are examples of convergent evolution. The order Sebacinales represents a sister group to the Agaricomycetes (Basidiomycota) that is comprised of ectomycorrhizal, ericoid-, orchid- mycorrhizal, root endophytic fungi and saprotrophs (Oberwinkler et al., 2013). Sebacinoid taxa are widely distributed from arctic to temperate to tropical ecosystems and are among the most common and species-rich groups of ECM, OM and endophytic fungi (Tedersoo et al., 2012, Tedersoo et al., 2010, Oberwinkler et al., 2013). The root endophyte Piriformospora indica and the orchid mycorrhizal fungus S. vermifera (MAFF 305830) are non-obligate root symbionts which were shown to be able to interact with many different experimental hosts, including the non-mycorrhizal plant Arabidopsis thaliana. These two fungi display similar colonization strategies in barley and in Arabidopsis and the ability to establish beneficial interactions with different hosts (Deshmukh et al., 2006). Colonization of the roots by P. indica and S. vermifera results in enhanced seed germination and biomass production as well as increased resistance against biotic and abiotic stresses in its experimental hosts, including various members of the Brassicaceae family, barley, Nicotiana attenuata and switchgrass (Ghimire, 2011, Ghimire et al., 2009, Ghimire et al., 2011, Waller et al., 2008, Barazani et al., 2007, Deshmukh et al., 2006). Microarray experiments were performed to identify and characterize conserved sebacinoid genes as key determinants in the Sebacinales symbioses.
2015-06-01 | GSE60736 | GEO
Project description:Fusarium oxysporum is an worldwide economically important plant fungi pathogen that can cause vascular wilt disease on a wide variety of hosts (Williamson et al., 2007). In recent years, extensive research has been conducted to interpret transcriptional regulation of virulence genes in FO. (Weiberg et al., 2013;Brandhoff et al., 2017;Wang et al., 2017;Wang et al., 2018;Porquier et al., 2019). However, gaps in the protein level studies limited deeper understanding of molecular basis of FO. pathogenesis. In this study, we conducted the first proteome-wide analysis in FO.
2023-07-17 | PXD043830 | Pride
Project description:Feng et al 2019 - Microarray Raw Data
| PRJNA562147 | ENA
Project description:Dichloromethane (DCM) extracts of aerial parts and roots of Waltheria indica analyzed in UHPLC-MS/MS in positive ionization mode. .raw, .mzML and MzMine2 processed files(spectra .mgf and feature table .csv) are available.
References:
Cretton, Sylvian, Stéphane Dorsaz, Antonio Azzollini, Quentin Favre-Godal, Laurence Marcourt, Samad Nejad Ebrahimi, Francine Voinesco, et al. 2016. “Antifungal Quinoline Alkaloids from Waltheria Indica.” Journal of Natural Products 79 (2): 300–307.
Cretton, S., L. Breant, L. Pourrez, C. Ambuehl, L. Marcourt, S. N. Ebrahimi, M. Hamburger, et al. 2014. “Antitrypanosomal Quinoline Alkaloids from the Roots of Waltheria Indica.” Journal of Natural Products 77 (10): 2304–11.
2021-12-06 | MSV000088521 | GNPS