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GNPS Retrospective Non-target Analysis to Support Regulatory Water Monitoring of Swiss Wastewater

ABSTRACT: Paper title: Retrospective Non-target Analysis to Support Regulatory Water Monitoring: from Masses of Interest to Recommendations via in silico workflows Author List: Adelene Lai, Randolph R. Singh, Lubomira Kovalova, Oliver Jaeggi, Todor Kondic, Emma L. Schymanski Brief description of the data submitted: Processed spectrum files in mzML format of Swiss wastewater samples measured using liquid-chromatography high-resolution tandem mass spectrometry (HR-MS/MS). Retrospective non-target analyses were performed on these data to tentatively identify chemical unknowns prioritised in regulatory monitoring.

INSTRUMENT(S): Q Exactive

ORGANISM(S): None.

SUBMITTER: Emma Schymanski

PROVIDER: MSV000086631 | GNPS | Tue Dec 22 11:07:00 GMT 2020

REPOSITORIES: GNPS

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Project description:Bio-augmentation could be a promising strategy to improve processes for treatment and resource recovery from wastewater. In this study, the Gram-positive bacterium Bacillus subtilis was co-cultured with the microbial communities present in wastewater samples with high concentrations of nitrate or ammonium. Glucose supplementation (1%) was used to boost biomass growth in all wastewater samples. In anaerobic conditions, the indigenous microbial community bio-augmented with B. subtilis was able to rapidly remove nitrate from wastewater. In these conditions, B. subtilis overexpressed nitrogen assimilatory and respiratory genes including NasD, NasE, NarG, NarH, and NarI, which arguably accounted for the observed boost in denitrification. Next, we attempted to use the the ammonium- and nitrate-enriched wastewater samples bio-augmented with B. subtilis in the cathodic compartment of bioelectrochemical systems (BES) operated in anaerobic condition. B. subtilis only had low relative abundance in the microbial community, but bio-augmentation promoted the growth of Clostridium butyricum and C. beijerinckii, which became the dominant species. Both bio-augmentation with B. subtilis and electrical current from the cathode in the BES promoted butyrate production during fermentation of glucose. A concentration of 3.4 g/L butyrate was reached with a combination of cathodic current and bio-augmentation in ammonium-enriched wastewater. With nitrate-enriched wastewater, the BES effectively removed nitrate reaching 3.2 mg/L after 48 h. In addition, 3.9 g/L butyrate was produced. We propose that bio-augmentation of wastewater with B. subtilis in combination with bioelectrochemical processes could both boost denitrification in nitrate-containing wastewater and enable commercial production of butyrate from carbohydrate- containing wastewater, e.g. dairy industry discharges. These results suggest that B. subtilis bio-augmentation in our BES promotes simultaneous wastewater treatment and butyrate production.

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GNPS Retrospective Non-target Analysis to Support Regulatory Water Monitoring of Swiss Wastewater

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