Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS Quantification_isobutanol_GCMS

ABSTRACT: Quantification of produced isobutanol by engineered E. coli strain

INSTRUMENT(S): Evolution3 Triple Quadrupole GC-MS/MS (Chromtech), 5975C Triple Quadrupole GC/MSD (Agilent Technologies)

ORGANISM(S): Escherichia Coli (ncbitaxon:562)

SUBMITTER: Steffen Klamt

PROVIDER: MSV000087056 | GNPS | Tue Mar 16 08:49:00 GMT 2021

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Similar Datasets

Genome-scale CRISPRi screen identifies pcnB repression conferring overproduction of free fatty acids in Escherichia coli

Project description:In this study, we carried out genome-scale CRISPRi modulation combining fluorescence-activated cell sorting (FACS) and next-generation sequencing (NGS) to identify genetic determinants for FFAs overproduction in Escherichia coli. The pcnBi (AP) strain that repressed the expression of pcnB produced 2992 mg l FFAs, which was 4.0-fold of the CF (A) strain. To analyze the underlying mechanism of FFAs overproduction, the engineered strain pcnBi and the control strain CF were applied to the transcriptomic and proteomic analyses.

2024-05-18 | PXD052390 | iProX

High-throughput Solexa sequencing of total RNA from Escherichia coli wild type and ∆fnr strain (PK4854)

Project description:Investigation of whole genome gene expression level changes in a Escherichia coli MG1655 K-12 ∆fnr mutant, compared to the wild-type strain. The mutations engineered into this strain produce a strain lacking the FNR protein.

2013-06-20 | GSE41190 | GEO

Expression analysis of Escherichia coli MG1655 K-12 WT and ∆arcA mutant

Project description:Investigation of whole genome gene expression level changes in a Escherichia coli MG1655 K-12 ∆arcA mutant, compared to the wild-type strain. The mutations engineered into this strain produce a strain lacking the ArcA protein. The results are further described in the manuscript The response regulator ArcA uses a diverse binding site architechture to globally regulate carbon oxidation in E. coli

2013-10-17 | GSE46412 | GEO

Expression analysis of Escherichia coli MG1655 K-12 WT and ∆fnr mutant

2013-06-20 | GSE41189 | GEO

Corynebacterium glutamicum as a powerful host for the production of 5-aminovalerate from glucose fermentation

Project description:5-aminovalerate (5AVA), L-lysine derived compound, represents a potential building block for the production of the bio-plastic nylon-5. Escherichia coli has been engineered for the production of 5AVA, but Corynebacterium glutamicum has never been engineered for the production of 5AVA, but, a lot of work was done in the last decades to optimize the production of the precursor L-lysine and more recently cadaverine. 5AVA added to the growth medium hardly affected growth rate of C. glutamicum, since, a half-inhibitory concentration of 1.1 M 5AVA was determined. While in E. coli, 5AVA production was engineered by using the DavBA pathway from Pseudomonas putida, here a pathway based on the route described in P. aeruginosa was established. C. glutamicum wild type was converted into a 5AVA producing strain by heterologous expression of L-lysine decarboxylase (LdcC), putrescine transaminase (PatA) and γ-aminobutyraldehyde dehydrogenase (PatD) genes from E. coli. 5AVA production was improved by using a strain previously engineered for high L-lysine production, by de-repressing phosphoenolpyruvate phosphotransferase system (PTS) and glycolysis and by avoiding formation of the by-product L-lactate. 5AVA accumulation by this strain was increased to 44.9 mM, representing a yield of 202 mmol mol-1 glucose, which is about three times higher than the highest yield achieved in E. coli for the production of 5AVA from glucose fermentation.

2016-06-17 | GSE83413 | GEO

Comparative study of extracellular proteins produced by Penicillium oxalicum strains

Project description:In order to study the effect of regulatory network engineering on the composition of extracellular proteins, the proteins produced by a engineered Penicillium oxalicum strain and its parent were identified and quantitified by LC-MS/MS.

2020-07-19 | PXD020433 | iProX

Systems analyses reveal the remarkable resilience of Escherichia coli physiology during accumulation and export of the non-native organic acid citramalate

Project description:Proteomics part of multi-omic analyses of the citramalate fermentation were undertaken to uncover the reasons for remarkable citramalate productivity in an engineered strain of E. coli.

2019-06-05 | PXD013088 | Pride

Gene expression by E. coli during the production of styrene and when exposed to externally added styrene

Project description:Transcriptomic studies of high-cell density fermentations of E. coli producing chemicals of relevance for biotechnology are limited. The aim of this study was to perform fermentations of an E. coli strain that had been engineered to produce styrene and examine the transcriptomic response. This was compared to an identical strain of E. coli that had the styrene pathway inactivated by the introduction of amino acid mutations in PAL2 and fdc1, the two enzymes required for styrene production. A further comparison was done by exposing E. coli cells to styrene added externally to the fermentation broth.

2022-03-12 | E-MTAB-8703 | biostudies-arrayexpress

Expression analysis of Escherichia coli MG1655 K-12 WT and M-bM-^HM-^Ffnr mutant

Project description:Investigation of whole genome gene expression level changes in a Escherichia coli MG1655 K-12 ?fnr mutant, compared to the wild-type strain. The mutations engineered into this strain produce a strain lacking the FNR protein. WT strains were grown under aerobic and anaerobic growth conditions. A six chip study using total RNA recovered from two separate cultures of Escherichia coli MG1655 K-12 WT (aerobic and anaerobic) and two separate cultures of the ?fnr mutant strain (anaerobic). Each chip measures the expression level of 4,661 genes from Escherichia coli MG1655 K-12 with eight 60-mer probes per gene, with each probe represented twice on the array.

2013-06-20 | E-GEOD-41189 | biostudies-arrayexpress

Expression analysis of Escherichia coli MG1655 K-12 WT and M-bM-^HM-^FarcA mutant

Project description:Investigation of whole genome gene expression level changes in a Escherichia coli MG1655 K-12 M-bM-^HM-^FarcA mutant, compared to the wild-type strain. The mutations engineered into this strain produce a strain lacking the ArcA protein. The results are further described in the manuscript The response regulator ArcA uses a diverse binding site architechture to globally regulate carbon oxidation in E. coli A six chip study using total RNA recovered from three separate cultures of Escherichia coli MG1655 K-12 WT and three separate cultures of the M-bM-^HM-^FarcA mutant strain. Each chip measures the expression level of 4,661 genes from Escherichia coli MG1655 K-12 with eight 60-mer probes per gene, with each probe represented twice on the array.

2013-10-17 | E-GEOD-46412 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data