Project description:Gene expression in Streptomyces turgidiscabies pathogenicity island was studied after transferring cells into thaxtomin A inducing medium OBB using Agilent 60mer oligonucleotide array with probes designed for S.scabies and S.turgidiscabies. Gene expression study was used to confirm results of gene prediction.
Project description:Gene expression in Streptomyces turgidiscabies pathogenicity island was studied after transferring cells into thaxtomin A inducing medium OBB using Agilent 60mer oligonucleotide array with probes designed for S.scabies and S.turgidiscabies. Gene expression study was used to confirm results of gene prediction. Time course gene expression experiment with two replicates
Project description:This study is aimed to isolate marine actinomycetes from sediments from Andaman and the Gulf of Thailand. All 101 marine actinomycetes were screened for anti-biofilm activity. Streptomyces sp. GKU223 showed significantly inhibited biofilm formation of S. aureus. The evaluation of supernatants of anti-biofilm activity produced by Streptomyces sp. GKU223 has been performed. Since the interaction between marine actinomycetes and biofilm forming bacteria has never been investigated, proteomic analysis has been used to identify whole cell proteins involved in anti–biofilm activity. Understanding the interaction at molecular level will lead to sustainably use for anti-biofilm producing marine actinomycetes in pharmaceutical and medicinal applications in the future.
Project description:This study is aimed to isolate marine actinomycetes from sediments from Andaman and the Gulf of Thailand. All 101 marine actinomycetes were screened for anti-biofilm activity. Streptomyces sp. GKU 257-1 showed significantly inhibited biofilm formation of E. coli. The evaluation of supernatants of anti-biofilm activity produced by Streptomyces sp. GKU 257-1 has been performed. Since the interaction between marine actinomycetes and biofilm forming bacteria has never been investigated, proteomic analysis has been used to identify whole cell proteins involved in anti–biofilm activity. Understanding the interaction at molecular level will lead to sustainably use for anti-biofilm producing marine actinomycetes in pharmaceutical and medicinal applications in the future.
Project description:In this study, we describe the isolation and identification of Streptomyces isolates collected from traditional medicinal plants’ rhizosphere during a campaign in Hamedan Province, Iran. Traditional medicinal plants represent a rich and unique source for the isolation of Streptomyces and new antimicrobial compounds. This strain was isolated from the rhizosphere of Helichrysum rubicundum
Project description:Human activities and climate change have negatively affected the world's oceans, leading to a 30-60% decline in biodiversity and habitats in coastal ecosystems. Marine turtles, as bioindicator species, accumulate contaminants, including trace elements, due to their extensive migration and long life span. However, there is a lack of data on the abundance of these contaminants and their effects on marine turtles' health. This study focuses on analyzing the muscle proteome of juvenile green sea turtles (Chelonia mydas) from Reunion Island. The ultimate goal was to evaluate whether muscle proteome responds to in-situ mixtures of inorganic contaminants to decipher the possible impacts on individual health, thereby identifying potential new biomarkers for long-term monitoring and conservation efforts.
Project description:Activating the cryptic secondary metabolic gene clusters is a vital research field in Streptomyces. The marine Streptomyces sp. FJNU027 strain which could produce tirandamycins was cultured in the oligotrophic medium. Compared with normal medium, a differential product in oligotrophic culture was found by HPLC assay. After mass fermentation, 2 mg of the differential product was obtained from 30 L fermentation broth by solvent extraction, column chromatography over sephadex LH-20 and reverse phase C18, and other methods. It was identified as 4,4',5,5'-tetramethyl-[1,1'- diphenyl]-2,2'-diol by NMR and MS data. The production of this compound was enhanced with the increment of cultural time. Transcriptome sequencing analysis showed that the highest upregulated genes under oligotrophic condition were glycosidase, TraR/DksA C4-type zinc finger protein and ribonuclease encoding genes, while the expression of a MarR family transcriptional regulator was most significantly decreased under oligotrophic condition. The results indicate that oligotrophic culture is an effective method for altering the secondary metabolism of Streptomyces.
Project description:HRMS guided study of 42 marine Streptomyces in the MAR4 clade. Metabolomics data collected from Ethyl Acetate crude extracts of 50 ml liquid cultures.
Project description:This study develops a pipeline for high-level production of the reverse antibiotic nybomycin from three seaweed species: Himanthalia elongata, Palmaria palmata, and Ulva lactuca. Screening Streptomyces strains identified S. explomaris, a marine species, as the best host to express the nybomycin gene cluster. The accumulated low yields in artificial seawater with brown seaweed hydrolysate. Gene expression analysis revealed downregulation of precursor supply pathways and upregulation of repressors, limiting biosynthesis. Metabolic engineering addressed these bottlenecks, leading to a superior S. explomaris mutant achieving 57 mg/L, a five-fold increase as compared to reported yields. The strain effectively valorized commercial seaweed hydrolysates, highlighting marine feedstocks' potential for antibiotic production.