Project description:M. hominis cells were grown in liquid medium supplied with arginine or thymidine as a carbon source. LC-MS analysis was performed on Ultimate 3000 Nano LC System (Thermo Fisher Scientific) coupled with Q Exactive HF benchtop Orbitrap mass spectrometer (Thermo Fisher Scientific) via a nanoelectrospray source (Thermo Fisher Scientific), an untargeted label-free bottom-up proteomic strategy was used, DDA (Data Dependent Acquisition) approach. Protein identification and label-free quantification were performed with PEAKS software.
Project description:We report a mass spectrometry-based proteomics approach to advance the study of the feline proteome. We analyzed immortalized feline cells, various tissues and plasma using data-dependent acquisition (DDA) on a Q Exactive HF mass spectrometer to develop a Felis Catus PeptideAtlas from 157 acquired runs. Data were analyzed using the Trans-Proteomic-Pipeline. We report in the first PeptideAtlas of the domestic cat the identification of 242,844 unique peptides representing 8,972 feline proteins or 45.6% of the predicted UniProtKB Felis catus proteome based on 19,655 unique genes and their representative protein sequences.
Project description:Untargeted metabolomics data of Stool samples run in Orbitrap Q Exactive. Data was acquired using UHPLC-MS/MS method, samples resuspended in 50-50 MeOH/H2O.
Project description:Plant metabolomic sample data. Contains .RAW and .mzXML formatted files. Data was acquired using data-dependent acquisition on a Thermo Q Exactive mass spectrometer.
Project description:Protein samples were immunoprecipitated using anti-HA beads. Eluates were separated by SDS-PAGE and Coomassie-stained, and target bands were excised for in-gel tryptic digestion after destaining, reduction, and alkylation. The resulting peptides were lyophilized and analyzed by LC-MS/MS on a Q Exactive mass spectrometer (ThermoFisher) coupled to an Easy nLC 1200 system. Peptides were separated on a C18 column using a 120-minute gradient. MS data were acquired in data-dependent acquisition mode with two replicates per condition.
Project description:Peptides of HCC cell lines and tumour tissues were fractionated by Hp-RP to eight fractions and repeated analysed three times by the LC-MS/MS detection system consisted of the EASY-nLC 1000 coupled to the Q-Exactive HF mass spectrometer using data dependent acquisition mode.
Project description:In this study, isobaric tags for relative and absolute quantification (ITRAQ) and Q Exactive mass spectrometer were used for non-targeted proteomic profiling of forearm arteries and veins in patients with chronic kidney disease stage 5 (with or without diabetes mellitus, obtained from arteriovenous fistula surgery) and healthy controls (obtained from patients with forearm trauma). Protein identifications were acquired against the Uniport Human database. As a result, 497609 spectra were obtained and 3244 proteins were identified with 96525 matched peptides.