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GNPS Heteropterys umbellata (Malpighiaceae) plant extracts

ABSTRACT: Analyses of Heteropterys umbellata (Malpighiaceae) stems and leaves extracts. Plants were collected in two different Brazilian States (Sao Paulo and Minas Gerais). Dried plant material was extracted with EtOH80%. Positive ionization mode analyses. LC-MS/MS performed in a Shimadzu HPLC using a Phenomenex C18-Luna (250 mm x 4.6 mm, 5.0 um) column and an Amazon SL (Bruker Daltonics) mass spectrometer (ion trap) equipped with an ESI source. Auto MSn mode for exploratory analysis.

INSTRUMENT(S): Amazon SL (Bruker Daltonics amaZon series)

ORGANISM(S): Heteropterys Umbellata

SUBMITTER: Vanderlan da Silva Bolzani

PROVIDER: MSV000089124 | GNPS | Thu Mar 24 13:31:00 GMT 2022

REPOSITORIES: GNPS

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Old Meets New: Mass Spectrometry-Based Untargeted Metabolomics Reveals Unusual Larvicidal Nitropropanoyl Glycosides from the Leaves of <i>Heteropterys umbellata</i>.

Mannochio-Russo Helena H Nunes Wilhan D G WDG Almeida Rafael F RF Albernaz Lorena C LC Espindola Laila S LS Bolzani Vanderlan S VS

Journal of natural products 20230227 3

The <i>Aedes aegypti</i> (Diptera: Culicidae) mosquito is the vector of several arboviruses in tropical and subtropical areas of the globe, and synthetic pesticides remain the most widely used combat strategy. This study describes the investigation of secondary metabolites with larvicidal activity from the Malpighiaceae taxon using a metabolomic and bioactivity-based approach. The workflow initially consisted of a larvicidal screening of 394 extracts from the leaves of 197 Malpighiaceae samples, ...[more]

PMID: 36848642

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Heteropterys umbellata (Malpighiaceae) plant extracts

2022-03-24 | MSV000089124 | MassIVE

Quantification and validation (MRM) of analysis - compounds isolated from Heteropterys umbellata (Malpighiaceae)

Project description:Analyses of Heteropterys umbellata (Malpighiaceae) stems and leaves extracts. Plants were collected in two different Brazilian States (Sao Paulo and Minas Gerais). Dried plant material was extracted with EtOH80%. Positive ionization mode analyses. LC-MS/MS analyses were performed in a Shimadzu HPLC using a Phenomenex C18-Luna (250 mm x 4.6 mm, 5.0 um) column and an Amazon SL (Bruker Daltonics) mass spectrometer (ion trap) equipped with an ESI source. Multiple reaction monitoring (MRM) mode for quantification of isolated compounds. The transitions 499.10->319.10, 501.10->365.10, 602.20->466.10, and 296.10->278.00 were chosen to monitor 3,5-di-O-caffeoylquinic acid (isochlorogenic acid A); 1,2,6-tris-O-[3-nitropropanoyl]-beta-glucopyranose (karakin); 1,2,3,6-tetrakis-O-[3-nitropropanoyl]-beta-glucopyranose; and sodium diclofenac (used as internal standard), respectively.

2022-04-30 | MSV000089350 | MassIVE

GNPS Quantification and validation (MRM) of analysis - compounds isolated from Heteropterys umbellata (Malpighiaceae)

2022-04-30 | MSV000089350 | GNPS

Gene expression analysis of multiple myeloma patients with relapse

Project description:Total RNA was obtained from bone marrow aspirates isolated from 16 multiple myeloma patients who underwent auto bone marrow transplantation (Auto-BMT). Our study was to define altered gene expression in MM relapse, gene expression profile was examined with 16 MM patients by progression-free survival (PFS) 12 months after autologous stem cell transplantation. Patients at each end of the PFS spectrum, 4 with shortest PFS and 4 with the longest PFS, were chosen for analyses to maximize the difference between different prognostic groups.

2016-07-06 | E-MTAB-4881 | biostudies-arrayexpress

Comparison of SND2-overexpressing and wild type Arabidopsis thaliana Col-0 inflorescence stems

Project description:Homozygous T4 SND2-overexpressing Arabidopsis plants were grown alongside the wild type and the lower 100 mm of primary inflorescence stems sampled at 4 and 8 weeks of age

2011-11-30 | GSE29693 | GEO

GNPS - Komagataella_phaffii_lipids_RP_IDX

Project description:LC-MS/MS and MSn (MS3 fragmentation in ion trap) lipidomics data of Komagataella phaffii recorded on an Orbitrap ID-X in positive ion mode (MSn) and positive and negative ion mode (MS/MS-files). For MSn files using exclusion and inclusion lists. Acquity HSS T3 (2.1 mm x 150 mm, 1.8 um, Waters) reverse phase column: A: ACN:H2O 6:4, 10 mM NH4Form, 0.1% FA, B: IPA:ACN 9:1, 10 mM NH4Form, 0.1% FA, 23 min gradient)

2022-07-29 | MSV000090011 | GNPS

Project description:Background: Tissue kallikrein-related peptidases 4, 5, 6 and 7 (KLK4-7) strongly increase the malignancy of ovarian cancer cells. By further deciphering the downstream effectors of KLK4-7, we aimed at finding new potential prognostic biomarkers and treatment targets for ovarian cancer patients. KLK4-7-transfected and vector-control OVMZ-6 (OV-KLK4-7 and OV-VC) ovarian cancer cells were established to select differentially regulated factors. Methods: Three independent approaches, PCR arrays, genome-wide microarray and proteome analyses, were pursued leading to the identification of ten candidates (MSN, KRT19, COL5A2, COL1A2, BMP5, F10, KRT7, JUNB, BMP4, MMP1). Differential gene expression in OV-KLK4-7 versus OV-VC cells was validated by qPCR. To determine differential protein expression, Western blot analyses, immunofluorescence and immunohistochemistry were applied for four candidates (MSN, KRT19, KRT7, JUNB) in both cell lines and tumor xenografts. Results: These experiments demonstrated that KLK4-7 distinctly regulate expression of MSN, KRT19, KRT7 and JUNB on the mRNA and protein levels in ovarian cancer cells and tissues. Protein expression of the top upregulated effectors, MSN and KRT19, was investigated by immunohistochemistry in a cohort of patients (n=66) afflicted with high-grade serous ovarian cancer and related to the immuno-expression levels of KLK4-7. Significant positive associations were found for KRT19/KLK4 (P=0.010), KRT19/KLK5 (P=0.030) and MSN/KLK7 (P=0.001); KRT19 and MSN immuno-expression showed a trend towards significance with KLK6. Conclusions: These findings imply that KLK4-7 exert key modulatory effects on other cancer-related genes and proteins in ovarian cancer. The key downstream effectors of KLK4-7, MSN and KRT19, may represent important therapeutic targets associated with serous ovarian cancer.

2018-12-26 | GSE109261 | GEO

GNPS - Malpighiaceae plants (9 species)

Project description:Analyses of 9 Brazilian Malpighiaceae species (leaves). Dried plant material were extracted, with EtOH80%. Positive and Negative ionization mode analises. LC-MS/MS performed in an Waters Acquity UPLC H-Class (Waters) using a Acquity BEH Shield RP18 reverse phase UHPLC column (1.7 um, 100 x 2.1 mm) and Xevo G2-XS QToF Mass Spectrometer (Waters) equipped with ESI source.

2020-03-19 | MSV000085121 | GNPS

NPAS4 controls cell type-specific circuit adaptations underlying drug-seeking behavior

Project description:Use of addictive substances often creates powerful and enduring associations with external cues that act as relapse triggers in individuals recovering from a substance use disorder (SUD). In the reward-associated brain region, the nucleus accumbens (NAc), drug use or drug-associated cue exposure activates a subset of D1 dopamine receptor-expressing medium spiny neurons (D1-MSNs), which typically promotes drug seeking, and a smaller subset of D2 dopamine receptor-expressing MSNs (D2-MSNs), which typically opposes drug seeking. The activity-regulated transcription factor, Neuronal PAS Domain Protein 4 (NPAS4), is activated in a small subset of NAc neurons during cocaine conditioning, and NAc NPAS4 is required for drug-context memories. Using a new Npas4-TRAP mouse combined with chemogenetics, we found that the during cocaine conditioning, the NPAS4-positive ensemble is required for drug-context associations. Single-cell transcriptomic analyses and in situ hybridization of NAc tissues from drug-conditioned mice revealed that NPAS4 is expressed predominantly in MSNs, and using cell type-specific molecular genetic approaches, we found that NPAS4 in D2-MSNs, but not D1-MSNs, was required for both drug-context associations and cue-reinstated cocaine seeking. Similarly, NPAS4 in NAc D2-MSNs, but not D1-MSNs, blocked cocaine experience-dependent strengthening of glutamatergic prefrontal cortical (PFC) inputs onto D2-MSNs. Analysis of differential gene expression in D2-MSNs revealed that NPAS4 and cocaine conditioning influence a gene expression program associated with synapses, dendrites, neuronal projections, dopamine, and cocaine. Together, our data reveal that NPAS4 functions during active cocaine use to maintain the imbalance of D1-MSN:D2-MSN activation and cue-induced drug seeking by suppressing excitatory drive onto relapse-opposing NAc D2-MSN circuits.

2023-08-31 | GSE210850 | GEO

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Project description:This study demonstrates the dichotomous activities of APRIL and BAFF in MM and DLBCL, which can be safely targeted by an engineered fusion protein designed to trap both ligands with ultrahigh binding affinity.

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