Dataset Information

GNPS - Relationship between thyroid hormones and associated metabolites and gene expression bioindicators in the serum of Rana [Lithobates] catesbeiana tadpoles and frogs during metamorphosis

ABSTRACT: Anuran metamorphosis is characterized by profound morphological changes including remodeling of tissues and organs. Here we investigate serum of Rana [Lithobates] catesbeiana (n=5-10) across seven distinct postembryonic stages beginning with premetamorphic tadpole (Gosner stage 31-33) and continuing through metamorphosis to a juvenile frog (Gosner stage 46). Using a sensitive nanoLC-Orbitrap system an untargeted analysis workflow was applied. Among 6,062 detected endogenous metabolites, 421 showed significant metamorphosis-dependent concentration dynamics. Among these potential bioindicators were several prostaglandins and other eicosanoids, some steroid hormones including testosterone, and several carnitines. These data provide a necessary context for interpreting developmental processes and potential molecular crosstalk mechanisms. Furthermore, this provides a solid foundation from which alternative bioindicators of metamorphosis may be chosen as feasible endpoints in future risk assessment of chemicals and their potency for causing developmental toxicity.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rana Catesbeiana (ncbitaxon:8400)

SUBMITTER: Martin Hansen

PROVIDER: MSV000090714 | GNPS | Tue Nov 15 04:46:00 GMT 2022

REPOSITORIES: GNPS

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Relationship between serum thyroid hormones and their associated metabolites, and gene expression bioindicators in the back skin of <i>Rana [Lithobates] catesbeiana</i> tadpoles and frogs during metamorphosis.

Poulsen Rikke R Jackman Shireen H SH Hansen Martin M Helbing Caren C CC

Frontiers in endocrinology 20230118

Anuran metamorphosis is characterized by profound morphological changes including remodeling of tissues and organs. This transition is initiated by thyroid hormones (THs). However, the current knowledge of changing levels of THs during metamorphosis relies on pooled samples using methods known for high variability with sparse reporting of measured variation. Moreover, establishing a clear linkage between key gene expression bioindicators and TH levels throughout the metamorphic process is needed ...[more]

PMID: 36743912

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