GNPS Guarana (Paullinia cupana) Josiel Amazonas_INPA-Brazil
Ontology highlight
ABSTRACT:
INSTRUMENT(S): micrOTOF-Q
ORGANISM(S): Paullinia Cupana Var. Sorbilis (ncbitaxon:392746)
SUBMITTER: 
Josiel do Nascimento Amazonas
PROVIDER: MSV000090891 | GNPS | Tue Dec 13 07:30:00 GMT 2022
REPOSITORIES: GNPS
Similar Datasets
Project description:The guarana extracts were submitted to liquid chromatography analysis coupled with mass spectrometry at the Analytical Center of the Thematic Laboratory of Chemistry of Natural Products-CALTQPN at INPA-Brazil. The extract samples obtained were prepared by weighing them in Eppendorf tubes and solubilizing them in a mixture of MeOH (0.1% formic acid) and 0.1% formic acid solution, in a 1:1 ratio, having the final concentration of 1 mg/mL. Finally, the tubes were centrifuged and transferred to vials for further analysis. The equipment used was a Liquid Chromatograph (Shimadzu, Japan) coupled to a high-resolution Time-of-flight mass spectrometer (MicroTOF QII model, Bruker Daltonics, Bremen, Germany), with an ESI-type ionization source in negative mode.
Luna5uPFP2;100A_15X2
Flx=0,1875 mL/minSSplit; P:1026 psi;F:30C;
C=1mg/mL(MeOH/H2O_1:1_0.1%ACF);Inj=10uL;
A(H2O+0.1%ACF)/B(ACN+0,1%ACF)HONEYWELL06/02
0-60_8-22%
60-62m_22-8%
62-70m _8%
injetor: MeOH/H2O_(0.1%ACF_1:1) (06-02-23)
Calib.:HCOONa 10mM_end
2023-04-15 | MSV000091719 | MassIVE
Project description:The guarana extracts were submitted to liquid chromatography analysis coupled with mass spectrometry at the Analytical Center of the Thematic Laboratory of Chemistry of Natural Products-CALTQPN at INPA-Brazil. The extract samples obtained were prepared by weighing them in Eppendorf tubes and solubilizing them in a mixture of MeOH (0.1% formic acid) and 0.1% formic acid solution, in a 1:1 ratio, having the final concentration of 1 mg/mL. Finally, the tubes were centrifuged and transferred to vials for further analysis. The equipment used was a Liquid Chromatograph (Shimadzu, Japan) coupled to a high-resolution Time-of-flight mass spectrometer (MicroTOF QII model, Bruker Daltonics, Bremen, Germany), with an ESI-type ionization source in negative mode. For analysis, a Luna 5u PFP2 100A column (15 cm x 2.00 mm) was used and the mobile phases with the solvents: A: 0.1% formic acid solution. MS/MS parameters: capillary: 3500 volts; Scan: 120-1200m/z; Set nebulizer: 2Bar psi; Set dry Heater: 180 Celsius; Set dry gas: 9.0 L/ min.; Rolling Averages: 1cts.
2023-02-07 | MSV000091217 | MassIVE
Project description:The guarana extracts were submitted to liquid chromatography analysis coupled with mass spectrometry at the Analytical Center of the Thematic Laboratory of Chemistry of Natural Products-CALTQPN at INPA-Brazil. The extract samples obtained were prepared by weighing them in Eppendorf tubes and solubilizing them in a mixture of MeOH (0.1% formic acid) and 0.1% formic acid solution, in a 1:1 ratio, having the final concentration of 1 mg/mL. Finally, the tubes were centrifuged and transferred to vials for further analysis. The equipment used was a Liquid Chromatograph (Shimadzu, Japan) coupled to a high-resolution Time-of-flight mass spectrometer (MicroTOF QII model, Bruker Daltonics, Bremen, Germany), with an ESI-type ionization source in negative mode. For analysis, a Luna 5u PFP2 100A column (15 cm x 2.00 mm) was used and the mobile phases with the solvents: A: 0.1% formic acid solution. MS/MS parameters: capillary: 3500 volts; Scan: 120-1200m/z; Set nebulizer: 2Bar psi; Set dry Heater: 180 Celsius; Set dry gas: 9.0 L/ min.; Rolling Averages: 1cts.
2023-03-08 | MSV000091435 | MassIVE
Project description:The guarana extracts were submitted to liquid chromatography analysis coupled with mass spectrometry at the Analytical Center of the Thematic Laboratory of Chemistry of Natural Products-CALTQPN at INPA-Brazil.
The extract samples obtained were prepared by weighing them in Eppendorf tubes and solubilizing them in a mixture of MeOH (0.1% formic acid) and 0.1% formic acid solution, in a 1:1 ratio, having the final concentration of 1 mg/mL. Finally, the tubes were centrifuged and transferred to vials for further analysis.
The equipment used was a Liquid Chromatograph (Shimadzu, Japan) coupled to a high-resolution Time-of-flight mass spectrometer (MicroTOF QII model, Bruker Daltonics, Bremen, Germany), with an ESI-type ionization source in negative mode. For analysis, a Luna 5u PFP2 100A column (15 cm x 2.00 mm) was used and the mobile phases with the solvents: A: 0.1% formic acid solution.
MS/MS parameters: capillary: 3500 volts; Scan: 120-1200m/z; Set nebulizer: 2Bar psi; Set dry Heater: 180 Celsius; Set dry gas: 9.0 L/ min.; Rolling Averages: 1cts.
2023-02-06 | MSV000091208 | MassIVE
Project description:Approximately 500 surface sterilized seeds of Arabidopsis seeds (ecotype: Col-0) were sterilely sown on filter disks overlaying solid ½ MS media 1% sucrose, stratified at 4C for 48 h and grown in darkness at 25C for 4 d. Seedlings were gently submerged by application to the plates of the different auxin solutions (made in ethanol carrier; 0.1% final concentration) and at the indicated times (20 min, 40 min, 60 min), the seedlings were lifted from the plate en mass and flash frozen in liquid nitrogen. Keywords: time-course
2003-10-12 | GSE664 | GEO
Project description:The aim of the project was to purify Xenopus replisome from replicationg chromatin assembled in Xenopus laevis egg extract. To this end recombinant Cdc45-TEV-His10-FLAG5 was expressed in bacteria and purified. 4 ml of Xenopus laevis egg extract was activated into interphase and supplemented with 10 ng/µl of demembranated sperm DNA, 70 nM recombinant Cdc45, 40 µM aphidicolin, 5 mM caffeine and incubated at 23°C for 60 min. Chromatin was isolated in ANIB100 buffer (50 mM HEPES pH 7.6, 100 mM KOAc, 10 mM MgOAc, 2.5 mM Mg-ATP, 0.5 mM spermidine, 0.3 mM spermine, 1 µg/ml of each aprotinin, leupeptin and pepstatin, 25 mM β-glycerophosphate, 0.1 mM Na3VO4 and 10 mM 2-chloroacetamide) as described previously (Gillespie, Gambus et al. 2012). Chromatin pellets re-suspended in ANIB100 containing 20% sucrose. Protein complexes were released from chromatin by digestion with 4 U/µl benzonase nuclease (E1014-25KU, Sigma) and sonicated for 5 min using a sonicator with settings: 30 sec on, 30 sec off, low power (Bioruptor Diagenode UCD-200). Immunoprecipitation was performed using 100 µl of anti-FLAG M2 magnetic beads (Sigma-Aldrich). Before elution the sample was washed four times with 1 ml of ANIB100 20% sucrose, ANIB100 20% sucrose 0.1% Triton X-100, ANIB100 and elution buffer (25 mM HEPES pH 7.5, 100 mM KAc, 5 mM MgAc, 1 mM ATP and 0.02% NP-40), respectively. The sample was eluted adding 250 µM 3xFLAG peptide (Stratech) to 200 µl of elution buffer and a small proportion of it analysed by mass spectrometry.
2023-10-27 | PXD044422 | Pride
Project description:The cells were lysed in a buffer consisting of 4% SDS, 0.1 M DTT, 100 mM Tris/HCl; pH 7.6 and heated for 5 minutes at 99℃. The protein extracts were processed according to theSp3 protocol. The reduced cysteine residues were alkylated in 200 mM iodoacetamide (Acros Organics). 20 μg of beads (1:1 mixture of hydrophilic and hydrophobic SeraMag carboxylate-modified beads; GE Life Sciences) were added to each sample in 50% ethanol. Protein clean-up was performed on a magnetic rack. The beads were washed twice with 80% ethanol followed by one wash with 100% acetonitrile (Fisher Chemical). The beads-captured proteins were digested overnight at 37 ℃ with 0.5 μg trypsin/LysC mix in 25 mM ammonium bicarbonate under vigorous shaking (1200 rpm, Eppendorf Thermomixer). The supernatants were collected and the peptides were purified by a modified Sp3 clean-up protocol and finally solubilized in the mobile phase A (0.1% formic acid in water), and sonicated. Peptide concentration was determined through absorbance measurement at 280 nm using a nanodrop instrument.
2024-05-08 | PXD047024 | Pride
Project description:The guarana extracts were submitted to liquid chromatography analysis coupled with mass spectrometry at the Analytical Center of the Thematic Laboratory of Chemistry of Natural Products-CALTQPN at INPA-Brazil. The extract samples obtained were prepared by weighing them in Eppendorf tubes and solubilizing them in a mixture of MeOH (0.1% formic acid) and 0.1% formic acid solution, in a 1:1 ratio, having the final concentration of 1 mg/mL. Finally, the tubes were centrifuged and transferred to vials for further analysis. The equipment used was a Liquid Chromatograph (Shimadzu, Japan) coupled to a high-resolution Time-of-flight mass spectrometer (MicroTOF QII model, Bruker Daltonics, Bremen, Germany), with an ESI-type ionization source in negative mode.
Luna5uPFP2;100A_15X2
Flx=0,1875 mL/minSSplit; P:1026 psi;F:30C;
C=1mg/mL(MeOH/H2O_1:1_0.1%ACF);Inj=10uL;
A(H2O+0.1%ACF)/B(ACN+0,1%ACF)HONEYWELL06/02
0-60_8-22%
60-62m_22-8%
62-70m _8%
injetor: MeOH/H2O_(0.1%ACF_1:1) (06-02-23)
Calib.:HCOONa 10mM_end
2023-04-15 | MSV000091719 | GNPS
Project description:Synechocystis 6803 cells was grown photoautotrophically at 32 °C buffered in BG-11 and bubbled with 3% CO2. A relatively mild Ci stress was applied by switching the aeration from 3% CO2 to air alone. After incubation under designated conditions, a 100-ml aliquot of culture was immediately combined with an equal volume of ice-cold mixture of phenol and ethanol (1:10, w/v) in an ice bath. The resultant cells were collected by centrifugation at 1000 x g for 10 min at 4 °C. Total RNA was isolated with RNeasy Midi Kit (Qiagen, Valencia, CA) and further treated with the DNA-free kit (Ambion, Austin, TX). Fluorescently labeled cDNA was produced via a two-step indirect procedure involving cDNA synthesis from 16 µg of total RNA in a reverse transcriptase reaction incorporating aminoallyl-modified deoxynucleotide, followed by the second step involving chemical coupling of fluorescent dye to the introduced amino moieties of the synthesized cDNA. Labeled cDNA were adjusted to 14.75 µl, and the remainder of the hybridization components containing 2.5 µl of 10 µg µl-1 salmon sperm DNA, 8.75 µl of 20x SSC, 0.25 µl of 10% SDS, and 8.75 µl of formamide were added. The mixture was then heated for 2 min at 99 °C and maintained at 42 °C until hybridization. Hybridizations were preformed in a static incubator at 42 °C for 12-16 h then washed by placing in a 250-ml solution of 2x SSC and 0.1% SDS at 42 °C for 5 min with gentle agitation provided by rotation of a magnetic stir bar. The slide was transferred quickly to a 250-ml solution of 0.1x SSC and 0.1% SDS, incubated for 10 min at room temperature with gentle agitation, and washed five additional times in 0.1x SSC for 1 min at room temperature. Hybridization signals from the microarray were quantified using GenePix Pro 4.1 (Axon Instruments, Union City, CA). The quality control procedures were conducted in the image analysis software, and then data were saved to Acuity 3.1 (Axon Instruments). Keywords: time-course
2004-08-24 | GSE1695 | GEO
Project description:For the MALDI-MSI experiment, we selected 12 different drugs. The drugs were purchased from the LC Laboratories (Woburn, MA; CAS numbers: dabrafenib: 1195765-45-7, dasatinib: 302962-49-8, erlotinib: 183321-74-6, gefitinib: 184475-35-2, imatinib: 152459-95-5, lapatinib: 388082-78-8, pazopanib: 444731-52-6, sorafenib: 284461-73-0, sunitinib: 557795-19-4, trametinib: 871700-17-3, vatalanib: 212141-54-3) and from SelleckChem (Munich, Germany; CAS numbers: ipratropium: 60205-81-4) with >99% purity and were dissolved in methanol (MeOH, (Chromasolv Plus for HPLC) (Sigma-Aldrich, Steinheim, Germany) at 10 mg/mL concentration. These stock solutions were further diluted with 50% MeOH and five mixtures were generated, each containing four different drug compounds. The spreadsheet in Supporting Information summarizes the composition of the five drug mixtures. A 5 mg/mL solution of α-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich) dissolved in 50% MeOH containing 0.1% trifluoroacetic acid (TFA, Sigma-Aldrich, Steinheim, Germany) was used as matrix solution.
2019-11-06 | PXD016146 | Pride