Dataset Information

GNPS - Lipid Exchange-Mass Spectrometry of AqpZ, AmtB, and AChR

ABSTRACT: Raw data for lipid exchange-mass spectrometry (LX-MS) experiments to profile lipidomic enrichment around E. coli AqpZ, AmtB, and T. californica AChR.

INSTRUMENT(S): Synapt XS

ORGANISM(S): Torpedo Californica Escherichia Coli (ncbitaxon:562) Sus Scrofa Domesticus (ncbitaxon:9825)

SUBMITTER: Michael Marty

PROVIDER: MSV000091960 | GNPS | Tue May 16 08:07:00 BST 2023

REPOSITORIES: GNPS

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Publications

Identifying Membrane Protein-Lipid Interactions with Lipidomic Lipid Exchange-Mass Spectrometry.

Zhang Guozhi G Odenkirk Melanie T MT Janczak Colleen M CM Lee Ray R Richardson Kevin K Wang Zhihan Z Aspinwall Craig A CA Marty Michael T MT

Journal of the American Chemical Society 20230912 38

Lipids can play important roles in modulating membrane protein structure and function. However, it is challenging to identify natural lipids bound to membrane proteins in complex bilayers. Here, we developed lipidomic lipid exchange-mass spectrometry (LX-MS) to study the lipid affinity for membrane proteins on a lipidomic scale. We first mix membrane protein nanodiscs with empty nanodiscs that have no embedded membrane proteins. After allowing lipids to passively exchange between the two populat ...[more]

PMID: 37700579

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