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GNPS - Lacinutrix shetlandiensis sp. nov. WUR7 extracts profiling

ABSTRACT: The MS was recorded by Dr. Giovanni Andrea Vitale in positive mode for all the ions in the mass range 150-1500 m/z, MS2 spectra were recorded for the most intense 5 ions mass in the range 150-1500 m/z, using a collision energy (CE): 40 eV, a scan rate: 10,000 Da/s, capillary voltage: 4.5 kV, source temperature: 200 C, declustering potential:150 V.

INSTRUMENT(S): QTRAP 4500

ORGANISM(S): Lacinutrix Shetlandiensis Sp. Nov. Wur7

SUBMITTER: Donatella de Pascale

PROVIDER: MSV000092280 | GNPS | Tue Jun 27 05:51:00 BST 2023

REPOSITORIES: GNPS

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A metabologenomics approach to unlock the metabolome of the novel Antarctic deep-sea isolate <i>Lacinutrix shetlandiensis</i> sp. nov. WUR7.

Vitale Giovanni A GA January Grant G GG Oppong-Danquah Ernest E Della Sala Gerardo G Palma Esposito Fortunato F Tasdemir Deniz D de Pascale Donatella D

PNAS nexus 20230706 7

The South Shetland Trough, Antarctica, is an underexplored region for microbiological and biotechnological exploitation. Herein, we describe the isolation and characterization of the novel bacterium <i>Lacinutrix shetlandiensis</i> sp. nov. WUR7 from a deep-sea environment. We explored its chemical diversity via a metabologenomics approach, wherein the OSMAC strategy was strategically employed to upregulate cryptic genes for secondary metabolite production. Based on hybrid de novo whole genome s ...[more]

PMID: 37448956

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Lacinutrix shetlandiensis sp. nov. WUR7 extracts profiling

Project description:The MS was recorded by Dr. Giovanni Andrea Vitale in positive mode for all the ions in the mass range 150-1500 m/z, MS2 spectra were recorded for the most intense 5 ions mass in the range 150-1500 m/z, using a collision energy (CE): 40 eV, a scan rate: 10,000 Da/s, capillary voltage: 4.5 kV, source temperature: 200 C, declustering potential:150 V.

2023-06-27 | MSV000092280 | MassIVE

Lacinutrix shetlandiensis sp. nov. WUR7 SPE-fractions DDA runs

Project description:The samples were analyzed by Dr. Ernest Oppong-Danquah, while molecular newtorking and chemoinformatic analysis were operated by Dr. Giovanni Andrea Vitale. The sample runs were executed in positive mode with the following conditions: capillary voltage: 3.0 kV, sample cone voltage: 30 V, source temperature: 150 C. A scan range from 50 to 1200 Da was used, MS2 fragmentation was achieved in DDA mode, with ramp collision energy: Low CE from 20_60 eV and a high CE of 40_80 eV.

2023-06-27 | MSV000092278 | MassIVE

GNPS - Lacinutrix shetlandiensis sp. nov. WUR7 SPE-fractions DDA runs

2023-06-27 | MSV000092278 | GNPS

28-days hindlimb unloading model mouse hippocampal synaptic proteomics

Project description:The peptide mixture was dissolved in water containing 0.1% formic acid (FA) and analyzed using a U3000-nano online system coupled with an Orbitrap Q-Exactive HFX mass spectrometer (Thermo Fisher Scientific, Massachusetts, USA).Data acquisition was performed in data-dependent mode. The MS scan range was set to an m/z of 350–1500 with an accumulation time of 0.25 s. The top 20 most intense ions in MS1 were selected for MS/MS analysis, with a dynamic exclusion time of 20 s.

2025-02-27 | PXD061253 |

Collected mass spectrometry data on monoterpene indole alkaloids from natural product chemistry research

Project description:MIADB: a cumulative collection of 172 tandem mass spectrometry (MS/MS) of a vast array of monoterpene indole alkaloids. Samples were analyzed using an Agilent LC-MS system composed of an Agilent 1260 Infinity HPLC coupled to an Agilent 6530 ESI-Q-TOF-MS operating in positive mode. A Sunfire analytical C18 column (150 × 2.1 mm; i.d. 3.5 μm, Waters) was used, with a flow rate of 250 μL/min and a linear gradient from 5% B (A: H2O + 0.1% formic acid, B: MeOH) to 100% B over 30 min. ESI conditions were set with the capillary temperature at 320 °C, source voltage at 3.5 kV, and a sheath gas flow rate of 10 L/min. The divert valve was set to waste for the first 3 min. There were four scan events: positive MS, window from m/z 100−1200, then three data-dependent MS/MS scans of the first, second, and third most intense ions from the first scan event. MS/MS settings were three fixed collision energies (30, 50, and 70 eV), default charge of 1, minimum intensity of 5000 counts, and isolation width of m/z 2. In the positive-ion mode, purine C5H4N4 [M + H]+ ion (m/z 121.050873) and the hexakis(1H,1H,3H-tetrafluoropropoxy)-phosphazene C18H18F24N3O6P3 [M + H]+ ion (m/z 922.009 798) were used as internal lock masses. Full scans were acquired at a resolution of 11 000 (at m/z 922). A permanent MS/MS exclusion list criterion was set to prevent oversampling of the internal calibrant.

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2021-07-19 | MSV000087853 | MassIVE

Expression data from HDFA cells exposed to nanosecond duration electrical pulses

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Expression data from U-937 cells exposed to nanosecond duration electrical pulses

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2016-02-16 | E-GEOD-77908 | biostudies-arrayexpress

Expression data from Jurkat Clone E-6 cells exposed to nanosecond duration electrical pulses

Project description:It is unclear how nanosecond electrical pulses affect gene expression. We used microarrays to identify how cells respond to this specific type of stress Jurkat cells were exposed to 100, 10 nanosecond duration pulses at 150 kV/cm. Gene expression was analyzed 4 hrs post exposure

2016-02-16 | E-GEOD-77907 | biostudies-arrayexpress

Acetaminophen (APAP) Rat Blood Training Gene Expression Data Set

Project description:Gene expression training data set from rat blood samples exposed to either 150, 1500 or 2500 mg/kg of APAP for 6, 12 or 24 hours. Keywords: Dose response, Time course, Microarray, Gene expression

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