Project description:Bulb, leaf, scape and flower samples of British bluebells (<i>Hyacinthoides non-scripta</i>) were collected regularly for one growth period. Methanolic extracts of freeze-dried and ground samples showed antitrypanosomal activity, giving more than 50% inhibition, for 20 out of 41 samples. High-resolution mass spectrometry was used in the dereplication of the methanolic extracts of the different plant parts. The results revealed differences in the chemical profile with bulb samples being distinctly different from all aerial parts. High molecular weight metabolites were more abundant in the flowers, shoots and leaves compared to smaller molecular weight ones in the bulbs. The anti-trypanosomal activity of the extracts was linked to the accumulation of high molecular weight compounds, which were matched with saponin glycosides, while triterpenoids and steroids occurred in the inactive extracts. Dereplication studies were employed to identify the significant metabolites via chemotaxonomic filters and considering their previously reported bioactivities.
Project description:We report the transcriptional changes associated with toxic effects of methanolic coal dust extract on normal zebrafish development. Early exposure of wild type embryos at 4 hpf to coal dust extract led to 3 groups of malformed phenotypes - tail deformity (P1), deformed yolk (P2) and smaller embryos with extruded yolks (P3). RNAseq of each phenotypic group revealed changes in genes involved in xenobiotic metabolism, intermediate filament composition, oxidation-reduction processes, calcium ion binding, focal adhesion and the ECM-receptor interaction pathway.
Project description:Molino, RJEJ., Rellin, KFB, Nellas, RB., Junio, HA. Small in size, big on taste: Metabolomics analysis of flavor compounds from Philippine garlic. PLoS ONE (2021) 16(5): e0247289. https://doi.org/10.1371/journal.pone.0247289
Project description:GNPS molecular networking analysis of 176- soil-derived fungi permitted a rapid prioritization of the fungus Penicillium shearii CMB-STF067 as a source of antibacterial active xanthoquinodin class of compounds, jugiones A-D.
Project description:This is the molecular network done using ddMS files of QC pool sample analyzed with C18 (positive, negative polarities) and Zic-Hilic (Positive, negative polarities). DMSP was identified in cold-adapted Antarctic Ulva and its holobiont, and several other polar compounds were up-regulated during heat stress.
The full dataset can be found under the MassIVE Accession MSV000097253
Project description:Amoroso, VB, Mendez, RA, Junio, HA., Molino, RJEJ, Pescadero, IR., Villalobos, AP. Characterization of a Natural Fungicide from an Indigenous Plant Tasmannia piperita (Hook.f.) Miers Extract: Stability, Metabolomics, and In silico Studies. Philippine Journal of Science (2021). 150 (2): 355-370
Collaboration between Secondary Metabolites Profiling Laboratory (SMPL), Institute of Chemistry, UP Diliman, and Central Mindanao University.