Project description:This project explores dietary proteins in human dental calculus through shotgun proteomics. These files are in addition to those accidentally not included in the original publication, Dairying enabled Early Bronze Age Yamnaya steppe expansions . DOI https://doi.org/10.1038/s41586-021-03798-4. Files include raw, mgf, and mzid files from the two Botai individuals: DA092 (Botai 2A), DA089 (CII(3) 30-40), and additional files from Russian sites: DA431, DA431 (Lebyazhinka 5, LEB N-0), Z333 (Khvalynsk 2, KHA2 N-12), and Z444 (Murziha 2, MUR2 N-128) as well as the blanks used in the experiments. It also inlcludes the corresponding files for DA436, as in the previous upload an under-injected sample was included as the MDF.
Project description:We report ATAC-seq for several A. thaliana accessions in healthy leaf tissue. As part of an investigation into the evolution of conserved noncoding sequences, our goal was to identify overlaps between regions of accessible chromatin and CNS. Manuscript in review. Biorxiv preprint doi: https://doi.org/10.1101/727669
Project description:To explore the link between Alzheimer's disease (AD) and obisity, we profiled the transcriptional changes of the co-morbidites as well as indivitual morbidities on brain, immune and adipose tissue, at the single cell level. We observed effects of both morbidities, including AD-associated alterations in microglia and astrocytes in AD mice, and HFD-associated transcriptional changes in teh brain and the adipose. See details in: doi: https://doi.org/10.1101/2022.02.05.479219
Project description:We compared the transcriptional changes in the cells of the corpus callosum, focusing on the oligodendrocyte and microglia, between LPC and cuprizone mediate demyelination. We further compared the transcriptional phenotypes to human MS patient samples. We identified distinct disease-associated oligodendrocyte states with shared pathological changes to MS, and observed a common but altered remyelination state between the models. Microglia response to demyelination is highly conserved, but human MS-associated microglia exhibit significantly more heterogeneity than we found in the mouse models. (doi: https://doi.org/10.1101/2025.03.24.645058)
Project description:Untargeted metabolomics data generated for the above publication for 13 lowland willows in Czech Republic and Austria. Extraction and methods are reported in Leong et al. (Accepted) and Sedio et al. 2021 Frontiers in Ecology and Evolution, https://doi.org/10.3389/fevo.2021.679638 [doi:10.25345/C59Z90N8R] [dataset license: CC0 1.0 Universal (CC0 1.0)].
Project description:This SuperSeries is composed of the SubSeries listed below. The main processed data are organized in the following two Figshare links: Seurat objects: https://doi.org/10.6084/m9.figshare.23290004 ReDeeM-V output: https://doi.org/10.6084/m9.figshare.24418966.v1
Project description:Processing (MzMine peak picking and generation of .mgf, quant files and Sirius ready .mgf) of the public MSV000085119 dataset. Check https://doi.org/doi:10.25345/C5S401 for further details on the original dataset.
Project description:Light triggers chloroplast differentiation whereby the etioplast transforms into a photosynthesizing chloroplast and the thylakoid rapidly emerges. However, the sequence of events during chloroplast differentiation remains poorly understood. Here we used whole-seedling proteome data to quantify changes in protein abundances during the course of de-etiolation within the first four days of light exposure. This data complements quantitative lipid and (ultra)structural data described in Pipitone et al. (doi: https://doi.org/10.1101/2020.08.30.274043).
Project description:We find that the 'high-confidence oscillating' genes initiate oscillations similarly as after release from L1 dauer. We performed high-throughput RNA sequencing on worms that were synchronously released from the dauer stage by refeeding on OP50. In particular, we investigate oscillatory gene expression as described in Hendriks et al., 2014 (doi: 10.1016/j.molcel.2013.12.013) and observe the re-initiation of oscillatory gene expression from stable expression with a time delay upon dauer exit. Experimental observation of the transitions between non-oscillatory and oscillatory states during the first 5 hours after dauer exit reveals that the oscillatory gene expression is arrested in a specific oscillator phase, similar to the observation we make in animals released from L1 arrest (see Meeuse et al., 2020, doi: https://doi.org/10.1101/755421).