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GNPS Mara1 Heterologous expression in S. albidoflavus J1074

ABSTRACT: Heterologous expression of Mara1 in S. ablidoflavus J1074. Mara1 is from S. mirabilis P8-A2

INSTRUMENT(S): 6545 Q-TOF LC/MS

ORGANISM(S): Streptomyces Albidoflavus (ncbitaxon:1886)

SUBMITTER: Ling Ding Karl Tilmann Weber

PROVIDER: MSV000093927 | GNPS | Fri Jan 26 04:11:00 GMT 2024

REPOSITORIES: GNPS

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Publications

Maramycin, a Cytotoxic Isoquinolinequinone Terpenoid Produced through Heterologous Expression of a Bifunctional Indole Prenyltransferase/Tryptophan Indole-Lyase in S. albidoflavus.

Maleckis Matiss M Wibowo Mario M Williams Sam E SE Gotfredsen Charlotte H CH Sigrist Renata R Souza Luciano D O LDO Cowled Michael S MS Charusanti Pep P Gren Tetiana T Saha Subhasish S Moreira José M A JMA Weber Tilmann T Ding Ling L

ACS chemical biology 20240514 6

Isoquinolinequinones represent an important family of natural alkaloids with profound biological activities. Heterologous expression of a rare bifunctional indole prenyltransferase/tryptophan indole-lyase enzyme from Streptomyces mirabilis P8-A2 in S. albidoflavus J1074 led to the activation of a putative isoquinolinequinone biosynthetic gene cluster and production of a novel isoquinolinequinone alkaloid, named maramycin (1). The structure of maramycin was determined by anal ...[more]

PMID: 38743035

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