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GNPS - Comprehensive Characterization of Extracellular Vesicles by Environmental (Neff) and Clinical (T4) Strains of Acanthamoeba castellanii

ABSTRACT: Proteomics, lipidomics, and metabolomics analysis of extracellular vesicles secreted by an environmental strain and a clinical strain of Acanthamoeba castellanii.

INSTRUMENT(S): LTQ Orbitrap Velos, 7890A GC coupled to a 5975C inert XL MSD, with a 7683 series injector (G2614A)

ORGANISM(S): Acanthamoeba Castellanii (ncbitaxon:5755)

SUBMITTER: Ernesto Nakayasu

PROVIDER: MSV000094477 | GNPS | Fri Apr 05 16:14:00 BST 2024

REPOSITORIES: GNPS

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Comprehensive characterization of extracellular vesicles produced by environmental (Neff) and clinical (T4) strains of <i>Acanthamoeba castellanii</i>.

Medeiros Elisa Gonçalves EG Valente Michele Ramos MR Honorato Leandro L Ferreira Marina da Silva MdS Mendoza Susana Ruiz SR Gonçalves Diego de Souza DdS Martins Alcântara Lucas L Gomes Kamilla Xavier KX Pinto Marcia Ribeiro MR Nakayasu Ernesto S ES Clair Geremy G da Rocha Isadora Filipaki Munhoz IFM Dos Reis Flavia C G FCG Rodrigues Marcio L ML Alves Lysangela R LR Nimrichter Leonardo L Casadevall Arturo A Guimarães Allan Jefferson AJ

mSystems 20240508 6

We conducted a comprehensive comparative analysis of extracellular vesicles (EVs) from two <i>Acanthamoeba castellanii</i> strains, Neff (environmental) and T4 (clinical). Morphological analysis via transmission electron microscopy revealed slightly larger Neff EVs (average = 194.5 nm) compared to more polydisperse T4 EVs (average = 168.4 nm). Nanoparticle tracking analysis (NTA) and dynamic light scattering validated these differences. Proteomic analysis of the EVs identified 1,352 proteins, wi ...[more]

PMID: 38717186

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Comprehensive Characterization of Extracellular Vesicles by Environmental (Neff) and Clinical (T4) Strains of Acanthamoeba castellanii

Project description:Proteomics, lipidomics, and metabolomics analysis of extracellular vesicles secreted by an environmental strain and a clinical strain of Acanthamoeba castellanii.

2024-04-05 | MSV000094477 | MassIVE

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Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles

Project description:A growing body of evidence in mammalian cells indicates that secreted vesicles can be used to mediate intercellular communication processes by transferring various bioactive molecules, including mRNAs and microRNAs. Based on these findings, we decided to analyze whether T. cruzi-derived extracellular vesicles contain RNA molecules and performed a deep sequencing and genome-wide analysis of a size-fractioned cDNA library (16–40 nt) from extracellular vesicles secreted by noninfective epimastigote and infective metacyclic trypomastigote forms. Our data show that the small RNAs contained in these extracellular vesicles originate from multiple sources, including tRNAs. In addition, our results reveal that the variety and expression of small RNAs are different between parasite stages, suggesting diverse functions. Taken together, these observations call attention to the potential regulatory functions that these RNAs might play once transferred between parasites and/or to mammalian host cells.

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sRNAs from Neisseria extracellular vesicles

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Impact of Group Psychological Interventions on Extracellular Vesicles in People Who Had Cancer

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mRNA data from extracellular vesicles secreted by Trichuris muris

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