Project description:This study investigated how gut-derived bacterial supplementation influences the honey bee gut microbiota, metabolism, and proteome under three experimental settings: (i) controlled laboratory conditions (C versus B), (ii) semi-controlled laboratory conditions with or without social interaction with older nestmates (COB versus BOB), and (iii) field conditions (CON versus TRT). A bacterial mixture containing Lactobacillus helsingborgensis, L. apis, Bifidobacterium choladohabitans, and B. polysaccharolyticum was administered. Gut samples were collected after 10 days and analyzed for microbiome (16s rRNA sequencing), metabolome (1H NMR), and proteome profiles (LFQ-proteomics, laboratory experiment only). Proteomic profiling revealed distinct group-specific host responses. Control bees (C and COB) upregulated ribosomal proteins linked to protein synthesis and cellular stress. Supplemented bees in group B showed strong induction of major royal jelly proteins (MRJP1 and MRJP5), associated with neural modulation and colony behavior. In BOB, elevated expression of mitochondrial enzymes suggested improved energy metabolism in the presence of social context. Bacterial supplementation induces reproducible, condition-dependent changes across microbiota, metabolic, and proteomic layers. These findings provide mechanistic insight into how microbial interventions reshape gut function and support honey bee health under realistic environmental conditions.
Project description:We performed scRNA-seq analysis on sympathetic ganglia, including the superior cervical ganglion (SCG), stellate ganglion (SG), and coeliac–superior mesenteric ganglion (CG-SMG). Comparative analysis of the transcriptomic profiling revealed molecularly distinct cell subtypes. We then performed scRNA-seq analysis in SCG and CG-SMG under control and stressed conditions (cold and microbiota-depletion). These analyses show that sympathetic ganglia undergo dynamic remodeling under stress, with distinct stressors driving ganglion-specific plasticity directly coupled to altered neuronal function.
Project description:This study applies proteomic stable isotope probing (proteomic SIP) to profile microbial protein synthesis during anaerobic incubation with 100% U ¹³C glucose. Cecal microbiota were cultured in deoxygenated M9 medium supplemented with 0.3 g L⁻¹ sodium thioglycolate, with U ¹³C glucose. Following incubation, microbial proteins were extracted, digested, and analyzed by nanoLC–MS/MS in DDA mode. Peptides (1 µg) were separated on an XSelect CSH C18 column over a 90 min gradient and acquired on an Orbitrap Fusion Tribrid. MS1 scans were collected at 120,000 resolution (m/z 375–1500); HCD MS2 scans at 30,000 resolution with a 5 m/z isolation window, 10 ppm isolation tolerance, and 20 s dynamic exclusion. Charge state specific NCE settings were used, and precursors with charge <+2, >+7, or unknown were excluded. The dataset enables evaluation of ¹³C glucose incorporation and supports metaproteomic characterization of active cecal taxa under anaerobic conditions.