Project description:Gigantol is a bioactive compound in Dendrobium officinale with a wide range of pharmacological properties. This study developed an ultrahigh-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) method for quantifying gigantol, which was subsequently applied to assess its pharmacokinetics and tissue distribution in Kunming mice. Additionally, the effects of gigantol on peroxisome proliferator-activated receptors (PPARs) were investigated. The UHPLC-Q-TOF/MS method was validated following the guidelines provided by the FDA and EMA for bioanalytical methods. Pharmacokinetic analysis revealed that gigantol exhibits a non-linear kinetic profile. Tissue distribution studies demonstrated high concentrations of gigantol in the liver and lungs. RNA sequencing (RNA-seq) indicated significant enrichment of PPAR signaling pathways in the KEGG database during gigantol-induced gene expression changes in the liver of Kunming mice. Molecular fingerprinting, docking studies and dual luciferase reporter assays confirmed that gigantol is a dual PPARα/γ agonist. These findings provide valuable insights for preclinical research on gigantol.
Project description:This LC-HRMS Data of Murraya paniculata was obtained from Orbitrap Eclipse Tribrid Mass Spectrometer with Nano LC and UHPLC and contains complex profile of bioactive compounds, including alkaloids, flavonoids, and coumarins.
Project description:Metabolic profiling from microalgal methanolic extracts using UHPLC-HRMS (Orbitrap Q Exactive). Dinoflagellate Alexandrium minutum were either healthy or infected with alveolate parasite Parvilucifera infectans, and cultivated under 20 PSU or 32 PSU. Samples were unpaired and extracted on Day 7, 14 and 21. The samples were processed using ZIC-HILIC chromatography to identify polar metabolites, followed by ddMS aquisition in the positive polarity mode. The supplementary files include, the study and result files from Compound Discoverer, the metadata table with normalization factor, sample code and conditions. The raw files of standards used confirm retention time and MS2 fragments of samples are included as well.