GNPS Human AKR1C3 Binds Agonists of GPR84 and Participates in an Expanded Polyamine Pathway (Pyrone Family Networking Analysis)
Ontology highlight
ABSTRACT:
INSTRUMENT(S): 6550 iFunnel Q-TOF LC/MS
ORGANISM(S): Escherichia Coli (ncbitaxon:562) Homo Sapiens (ncbitaxon:9606)
SUBMITTER: 
Jason Crawford
PROVIDER: MSV000095067 | GNPS | Tue Jun 18 09:40:00 BST 2024
REPOSITORIES: GNPS
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Project description:Representative tandem MS files generated from mouse type I fatty acid synthase biochemical assay with NADPH titration.
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File description:
in-vitro-NADPH-0-1msms: a representative tandem MS file of alpha pyrones from mFAS in vitro biochemical assay (as described above) titrated with 0 uM NADPH co-factor.
in-vitro-NADPH-100-1msms: a representative tandem MS file of alpha pyrones from mFAS in vitro biochemical assay (as described above) titrated with 100 uM NADPH co-factor.
in-vitro-NADPH-200-1msms: a representative tandem MS file of alpha pyrones from mFAS in vitro biochemical assay (as described above) titrated with 200 uM NADPH co-factor.
Reversed-phase chromatography was performed with a Kinetex (Cat. # 00G-4601-E0) 5 um C18 100 A column (250 by 4.6 mm), using a water:acetonitrile gradient containing 0.1% formic acid at 0.7 mL/min flow rate: 0-30 min, 10% to 100% acetonitrile. Positive mode (qTOF)
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