Project description:This project includes water samples that were collected in West Maui, Hawaii, USA following the August 2023 wildfires. The majority of samples were collected along the shoreline, both inside and outside of the burn zone. A subset of samples were collected along an offshore transect. Another subset of samples were collected in or at the mouth of streams within the burn zone. All water samples consisted of 200 ml of water, pre-filtered with 0.2 um sterivex, run over Agilent HLB columns, and eluted with methanol.
Project description:Non-targeted LC-MS/MS analysis of PPL solid phase extracted dissolved organic matter (DOM) from TARA/TREC Expedition Leg 1, collected in the coastal Atlantic between France to Netherlands in Spring 2023.
Project description:Marine cyanobacteria are thought to be the most sensitive of the phytoplankton groups to copper toxicity, yet little is known of the transcriptional response of marine Synechococcus to copper shock. Global transcriptional response to two levels of copper shock was assayed in both a coastal and an open ocean strain of marine Synechococcus using whole genome expression microarrays. Both strains showed an osmoregulatory-like response, perhaps as a result of increasing membrane permeability. This could have implications for marine carbon cycling if copper shock leads to dissolved organic carbon leakage in Synechococcus. The two strains additionally showed a reduction in photosynthetic gene transcripts. Contrastingly, the open ocean strain showed a typical stress response whereas the coastal strain exhibited a more specific oxidative or heavy metal type response. In addition, the coastal strain activated more regulatory elements and transporters, many of which are not conserved in other marine Synechococcus strains and may have been acquired by horizontal gene transfer. Thus, tolerance to copper shock in some marine Synechococcus may in part be a result of an increased ability to sense and respond in a more specialized manner.