Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS - Inducible antimicrobial activity in Bacillus spp and 2-PBI confirmation

ABSTRACT: Follow up confirmation of induced metabolites in bacterial cultures. Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 1.7 um 100 A pore size C18 reversed phase UHPLC column 100 x 2.1 mm (Phenomenex, Torrance, CA).

INSTRUMENT(S): Orbitrap Exploris 240

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. Ncib 3610 (ncbitaxon:535026) Bacillus Cereus (ncbitaxon:1396)

SUBMITTER: Valeska Villegas

PROVIDER: MSV000095664 | GNPS | Tue Aug 20 16:57:00 BST 2024

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

GNPS - Inducible antimicrobial activity in Bacillus spp

Project description:In previous research, we have discovered a phenomenon of inducible antagonistic activity in strains of the order Bacillales in which, large inhibition zones were produced against different pathogens when growing in the presence of the chemical compound triphenyl tetrazolium chloride (TTC), where Bacillus subtilis NCIB3610, Bacillus cereus EA-CB1047 and B. tequilensis EA-CB0015 how high activity against the phytopathogen Ralstonia solanacearum. The exact structure of these compounds has not been so far elucidated; therefore, this project aims to clarify whether the changes in the metabolic profiles of Bacillus sp. correspond to biotransformation reactions of TTC or might be products of any additional biosynthetic process not yet revealed, using molecular networks approaches.

2022-12-12 | MSV000090888 | GNPS

GNPS - Eelgrass Diversity Mesocosm Experiment

Project description:Follow up study. This project was conducted in collaboration with the Stachowicz and Dorrestein labs exploring the effects of eelgrass genotypic diversity on rhizosphere exometabolome production under marine heat wave versus ambient conditions. Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 1.7 um 100 A pore size C18 reversed phase UHPLC column 100 x 2.1 mm (Phenomenex, Torrance, CA).

2025-05-20 | MSV000097940 | GNPS

Metabolomic analysis of an inducible antimicrobial activity in Bacillus spp. by molecular networking

2022-04-28 | MSV000089329 | MassIVE

GNPS - SDZWA Elephant and Rhino Milk Untargeted LC-MSMS NEG

Project description:Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 2.6 um 100 A pore size PolarC18 reversed phase UHPLC column 100 x 2.1 mm (Phenomenex, Torrance, CA).

2024-12-05 | MSV000096600 | GNPS

GNPS - SDZWA Elephant and Rhino Milk Untargeted LC-MSMS POS

2024-12-05 | MSV000096599 | GNPS

GNPS - Algal induction from a spotted salamander host dataset 2 NEG

Project description:Follow up study. Samples corresponding to sterile filtered metabolites from before and after the bloom period of development of blastopore of neurulating Ambystoma maculatum (spotted salamander) hosts. Algal species (Oophila amblystomatis) bloom outside the blastopore of the host. Pond water and rearing media samples are also included. Untargeted LC-MS/MS acquisition (negative mode) was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 2.6 um 100 A pore size Polar C18 reversed phase UHPLC column 100 x 2.1 mm (Phenomenex, Torrance, CA).

2025-05-06 | MSV000097814 | GNPS

GNPS - Algal induction from a spotted salamander host dataset 2 POS

Project description:Follow up study. Samples corresponding to sterile filtered metabolites from before and after the bloom period of development of blastopore of neurulating Ambystoma maculatum (spotted salamander) hosts. Algal species (Oophila amblystomatis) bloom outside the blastopore of the host. Pond water and rearing media samples are also included. Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 2.6 um 100 A pore size Polar C18 reversed phase UHPLC column 100 x 2.1 mm (Phenomenex, Torrance, CA)

2025-05-06 | MSV000097813 | GNPS

Gluten hydrolyzing activity of Bacillus spp isolated from sourdough.

Project description:BackgroundCeliac disease is an intestinal chronic disorder with multifactorial etiology resulting in small intestinal mucosal injuries and malabsorption. In genetically predisposed individuals with HLA DQ2/DQ8 molecules, the gluten domains rich in glutamine and proline present gluten domains to gluten reactive CD4+ T cells causing injury to the intestine. In the present experimental design, the indigenous bacteria from wheat samples were studied for their gluten hydrolyzing functionality.ResultsProteolytic activity of Bacillus spp. was confirmed spectrophotometrically and studied extensively on gliadin-derived synthetic enzymatic substrates, natural gliadin mixture, and synthetic highly immunogenic 33-mer peptide. The degradation of 33-mer peptide and the cleavage specificities of the selected isolates were analyzed by tandem mass spectrometry. The gluten content of the sourdough fermented by the chosen bacterial isolates was determined by R5 antibody based competitive ELISA. All the tested isolates efficiently hydrolyzed Z-YPQ-pNA, Z-QQP-pNA, Z-PPF-pNA, and Z-PFP-pNA and also cleaved 33-mer immunogenic peptide extensively. The gluten content of wheat sourdough was found to be below 110 mg/kg.ConclusionIt has been inferred that four Bacillus spp especially GS 188 could be useful in developing gluten-reduced wheat food product for celiac disease prone individuals.

| S-EPMC7291523 | biostudies-literature

Homo sapiens whole blood infected with Candida spp. (here: Candida parapsilosis)

Project description:Homo sapiens fresh whole blood was infected with Candida parapsilosis. RNA-pool of both species extracted at 0min (control), 15, 30, 60, 120, 240 min. Samples are rRNA depleted. Measurement of Homo sapiens gene expression.

2018-12-26 | GSE114177 | GEO

Homo sapiens whole blood infected with Candida spp. (here: Candida glabrata)

Project description:Homo sapiens fresh whole blood was infected with Candida glabrata. RNA-pool of both species extracted at 0min (control), 15, 30, 60, 120, 240 min. Samples are rRNA depleted. Measurement of Candida glabrata gene expression.

2018-12-26 | GSE114175 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data