Project description:Select species were grown in Brain-Heart-Infusion supplemented with 1 mM cholic acid and 0.1 mM taurine for 16 hours. Data are in positive ion mode.
Project description:Sponge samples were analyzed on the QExactive in positive ESI mode without and with metal infusions (ca, vanadium, mg) and (fe, cu, zn).
Project description:Use CEN quechers method to extract 2.5g honey and use PSA to clean up matrix. Using ACN-water-0.01%HCOOH for compound separation and HRMS analysis with Thermo Orbitrap Exploris 120. Data was acquired in Full scan-ddms2 mode. This included a full scan over the m/z range 100- 1000 at full width at half maximum (FWHM) resolution of 60,000, and a data-dependent-MS2 scan at FWHM resolution of 15,000 on the top 4 ions. The ionization was performed in positive ESI with an inlusion list collated from OPPIN website, and to gain more information about fragment ions in the QC sample, we use an Automated Exclusion List Generation workflow, so one QC sample finally gave 3 injections.