Project description:Twenty microliter aliquots of BALF samples were dissolved in SDS-sample buffer and applied onto a 4-12% Nupage gel with MOPs running buffer. The run stopped after the samples migrated approximately ¼ distance into the gel. Each lane of the gel was sliced into smaller pieces, and subjected to destaining, reducing/alkylation, and in-gel trypsin digestion. The extracted peptides were applied for LC-MS/MS analysis using either a Thermo Orbitrap Fusion or a Thermo Orbitrap Fusion Lumos operated with an in-line Thermo nLC 1200 and an EASY-Spray ion source. Pepetides were separated using a 2 cm Pepmap 100 C18 trap column and a 25 cm Easy-spray Pepmap 100 C18 analytical column. MS/MS data acquisitions were operated at a 120,000 resolution (m/z 200) with a scan range of 350-1950 m/z and CID fragmentation.
Project description:MS/MS fragmentation data of foodbiomarker standards acquired on Q Exactive - with
chromatographic separation on a Phenomenex polar C18 column.
Project description:MS/MS fragmentation data of coffee arabica samples acquired on QTOF with chromatographic separation on a phenomenex polar C18 column
Project description:MS/MS fragmentation data of sample VD_C13 acquired on Q Exactive - with chromatographic separation on a Phenomenex polar C18 column.
Project description:MS/MS fragmentation data of synthetic standards acquired on Q Exactive - with chromatographic separation on a Phenomenex polar C18 column.
Project description:MS/MS fragmentation data of Reaction was acquired on Q Exactive - with
chromatographic separation on a Phenomenex polar C18 column.
Project description:MS/MS fragmentation data of bile acids acquired on timsTOF pro2 - with
chromatographic separation on a Phenomenex polar C18 column.
Project description:MS/MS fragmentation data of lizard samples acquired on Q Exactive - with
chromatographic separation on a Phenomenex polar C18 column.