Metabolomics

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GNPS Co-Purified His-Rich Putative Nickel Insertion Protein larC_Band in Gel


ABSTRACT: An unknown protein co-purified during HisTrap affinity purification from Haloferax volcanii cell lysate grown in glucose minimal medium. The protein was initially present in a buffer containing 50 mM HEPES and 2 M NaCl. The sample was then resuspended in 2x Laemmli sample buffer and resolved by SDS-PAGE using 1x SDS running buffer. Following electrophoresis, the gel was fixed and stained overnight with Bio-Safe Coomassie. The resulting band corresponding to the unknown protein was subsequently excised for analysis.

INSTRUMENT(S): Q Exactive HF Orbitrap

ORGANISM(S): Haloferax Volcanii Ds2 (ncbitaxon:309800)

SUBMITTER: Julie A. Maupin-Furlow  

PROVIDER: MSV000097920 | GNPS | Mon May 19 21:12:00 BST 2025

SECONDARY ACCESSION(S): PXD064085

REPOSITORIES: GNPS

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