Project description:Batch2 includes WT, ko, and OE lines along with other treatments of AOA, coculture etc, under Fe sufficient and deficient conditions.
Project description:We conducted iTRAQ analyses using soluble proteins extracted from roots of non-transformant rice (NT) and previously generated HRZ-knockdown (HRZi) lines (Kobayashi et al., 2013 Nat Commun 4: 2792) cultivated under Fe-sufficient and Fe-deficient conditions. We identified and quantified 3,033 and 3,525 proteins in replicate 1 and 2, respectively, which totaled 4,884 proteins including 1,698 overlapping proteins between the two replicate experiments.
Project description:We report the comparison of transcript expression of Fe-sufficient roots of OsIMA overexpression lines IMA1OX-4, IMA2OX-1, OsIMA knockdown lines IMA1i-2, IMA2i-3, and corresponding NT, as well as Fe-deficient roots of NT, by RNA-Seq.
Project description:To study the regulation of Lepr on transcriptome in WT and Lepr KO AMs under resting state and after LPS stimulation, Lepr-sufficient (Lepr+/+, Lyz2-Cre) and Lepr-deficient (Leprfl/fl, Lyz2-Cre) alveolar macrophages (AMs) were isolated by collecting BALF. Lipopolysaccharide (LPS) was added in vitro for 1h or cells were left untreated. Total RNA was extracted for deep sequencing. Gene expression in WT and Lepr KO cells were analyzed.
Project description:We performed single cell transcriptome analysis of Treg cells (CD4+CD25+) from healthy Mycfl/flFoxp3Cre/WT female mice, in which Myc-sufficient (WT) and Myc-deficient (KO) Treg cells co-exist due to random inactivation of the X chromosome that harbors the Foxp3 gene.
Project description:Dictyostelium discoideum O-Ser-fucosylated proteins from WT and SPY-KO vegetative cells were immunoprecipitated using anti O-Ser fucose polyclonal antibodies in 3 Biological Replicates, with 3 to 6 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).
Project description:Dictyostelium discoideum O-Thr-fucosylated proteins from WT and SPY-KO vegetative cells were immunoprecipitated using anti O-Thr fucose polyclonal antibodies in 3 Biological Replicates, with 3 to 6 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).
Project description:Dictyostelium discoideum O-Ser-fucosylated proteins from WT and SPY-KO developing cells (slugs) were immunoprecipitated using anti O-Ser fucose polyclonal antibodies in 3 Biological Replicates, with 3 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).
Project description:Dictyostelium discoideum O-Thr-fucosylated proteins from WT and SPY-KO developing cells (slugs) were immunoprecipitated using anti O-Thr fucose polyclonal antibodies in 3 Biological Replicates, with 3 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).
Project description:Transcriptional profiling of Yellow stripe 1 (ys1) and ys3 mutants. ys1 and ys3 are recessive mutants of maize (Zea mays L.) that result in symptoms typical of Fe deficiency, i.e., interveinal chlorosis of the leaves. The objective of the present work was to identify the genes involved in the ys1 and ys3 phenotypes, so as to extend our understanding of Fe homeostasis in maize. Root or shoot of WT vs. ys1 or ys3 mutants under Fe sufficient or Fe deficient conditions respectively.