Project description:The goal of this study is to characterize the human immune responses to the live attenuated Herpes zoster vaccine Zostavax, to understand the molecular and cellular mechanisms that lead to antibody production and T cell induction, and to understand the difference between young and elderly healthy adults. The overall data collection included antigen specific assays, flow cytometric profiling of innate and adaptive cell populations, measurement of serum cytokines, and transcriptomic and metabolomics signatures. Zostavax induced robust antigen-specific antibody responses, and significant T cell responses. A number of gene pathways were upregulated after vaccination. Using our previously developed blood transcription modules, we also identified transcriptomic correlates to antibody response. Furthermore, this study revealed strong association between PBMC transcriptomics and plasma metabolomics. Integrative analysis of orthogonal datasets from metabolomics, transcriptomic and immune profiling facilitated a temporal reconstruction of Zostavax induced biological networks culminating in antibody responses , and the delineation of novel molecular correlates of vaccine immunity.
Project description:Bulk high-resolution mass spectrometry can provide sensitive and global snapshots of metabolites involved in cancer metabolism. However, intra-tumor heterogeneity (IntraTH) convolutes the cellular origins and tumor pathologies associated with the detected metabolites, thus making the elucidation of reproducible metabolic pathways and/or biomarkers very challenging. Here, we present “Spatially-guided MEtabolomics (SgME) profiling”, a multi-modal metabolomics data analysis approach to delineate IntraTH by integrating spatial and bulk metabolomics profiles from the same tumors. We applied SgME profiling to 117 tumor and adjacent normal tissues from 26 surgically resected primary liver tumors, and constructed SgME maps of metabolic regions (MERs) associated with key histopathological features. We used these maps to survey IntraTH and train regression models that accurately predict the MER compositions of bulk tumor samples. We also discovered a group of putative metabolites that increase in low-grade tumor regions but abruptly decrease in necrotic regions. SgME profiling may also be applied to heterogeneous tissues from other cancer types or metabolic diseases and provide systems-level understandings of the roles of local cellular niches in cancer metabolism and tumorigenesis.
Project description:untargeted metabolomics (RPLC, negative mode) on human milk samples to investigate the presence of maternal drugs and dietary factors in breast milk
Project description:untargeted metabolomics (RPLC, positive mode) on human milk samples to investigate the presence of maternal drugs and dietary factors in breast milk
Project description:This study aims at identifying a dual transcriptomics and metabolomics blood signature following administration of CpG-ODN (cytosine-phosphate-guanine oligodeoxynucleotides), a reference immune-stimulatory molecule. A clinical study was conducted with chicks and transcriptomics and metabolomics analyses were performed on whole-blood and plasma samples respectively. Statistical analyses resulting in lists of differentially expressed genes and metabolites with different abundance were identified in chicks treated with CpG-ODN. The results showed that CpG-ODN activates the innate immune systems within hours following administration and its effect lasts over time, as metabolomic and transcriptomic profiles are still varying at 6 days after administration.
Project description:To characterize the human plasma microtranscriptome profile at first trimester of pregnancy in presence or not of pregnancy complications, we sequenced microRNAs in plasma samples collected from pregnant women between the 4th and the 16th weeks of pregnancy. We then performed differential expression analyses to assess the miRNA profile diffrences according to the presence of pregnancy complications or not (i.e. Gestational diabetes mellitus, Gestational hypertension or preeclampsia vs. normal pregnancies).
Project description:Interventions: Group 1:Routine therapy plus Xiangbin prescription for patients with colorectal cancer (preoperative + postoperative);Group 2:Routine therapy plus placebo for patients with colorectal cancer (preoperative + postoperative);Healthy person:No Intervention
Primary outcome(s): Small Molecular Metabolomics of Blood
Study Design: Non randomized control