Project description:Triple-negative breast cancer (TNBC) presents a significant challenge in women’s health due to its aggressive phenotype and the absence of targeted therapeutic options. β-Elemene, a sesquiterpene isolated from Curcuma wenyujin, has demonstrated clinical benefits against TNBC; however, its mechanisms of action, particularly with respect to the immune and metabolic tumor microenvironment, remain poorly characterized. In this study, we employed single-cell RNA sequencing and untargeted metabolomics to investigate how β-elemene reshapes the cellular and metabolic landscape of TNBC using a 4T1 orthotopic mouse model. Our findings are expected to provide the first comprehensive elucidation of β-elemene’s dual immunomodulatory and metabolic effects in TNBC, highlighting the potential of natural compounds to enhance antitumor immunity.
Project description:We isolated single cells (using either liberase or collagenase A dissociation) from subcutaneous adipose tissue collected from abdominoplasty surgery, and healthy liver tissue adjacent to tumor from liver resection and ran 10X v2 3' scRNA Seq. We ran 3 channels for adipose (IRB 2007P002165/1(ORSP-3877)) and 4 channels for liver (IRB 02-240; ORSP 1702 and ORSP-2630 under ORSP-2169). Channels were merged and uploaded here. *Raw data files absent*
Project description:We analyzed polar and non-polar solvent extracts of yeast with an Exploris 240 mass spectrometer and used AcquireX software for deep interrogation of compounds.