A Coordinated Proteomic Approach for Identifying Proteins that Interact with the E. coli Ribosomal Protein S12.

ABSTRACT: Data from PASSEL: [[https://db.systemsbiology.net/sbeams/cgi/PeptideAtlas/PASS_View?datasetPassword=IR4769n&identifier=PASS00136 PASS00136]]. Experiment: 1st exerimental NAc pQ MeS, data file: F571953.mgf. Data published as part of J Proteome Res. 2013 12:1289-99 . From the Abstract: {{i}} ... To follow-up on this study, the D88 containing loop was probed to identify candidate binders employing a two-step complementary affinity purification strategy. The first involved an endogenously expressed S12 protein containing a C-terminal tag for capturing S12 binding partners. The second strategy utilized a synthetic biotinylated peptide representing the D88 conserved loop region for capturing S12 loop interaction partners. Captured proteins from both approaches were detected by utilizing SDS-PAGE and one-dimensional liquid chromatography-tandem mass spectrometry. ...{{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Escherichia_coli_k_12_substr__dh10b, Escherichia_coli_k_12_substr__mg1655, Enterobacteria_phage_13a, Enterobacteria_phage_933w, Enterobacteria_phage_alpha3, Enterobacteria_phage_ba14, Enterobacteria_phage_bp_4795, Enterobacteria_phage_bz13, Enterobacteria_phage_cdti, Enterobacteria_phage_ecods1, Enterobacteria_phage_eps7, Enterobacteria_phage_epsilon15, Enterobacteria_phage_es18, Enterobacteria_phage_felix_01, Enterobacteria_phage_fels_2, Enterobacteria_phage_fi_sensu_lato, Enterobacteria_phage_g4_sensu_lato, Enterobacteria_phage_hk022, Enterobacteria_phage_hk620, Enterobacteria_phage_hk97, Enterobacteria_phage_i2_2, Enterobacteria_phage_id18_sensu_lato, Enterobacteria_phage_id2, Enterobacteria_phage_if1, Enterobacteria_phage_ike, Enterobacteria_phage_jk06, Enterobacteria_phage_js10, Enterobacteria_phage_js98, Enterobacteria_phage_jse, Enterobacteria_phage_k1e, Enterobacteria_phage_k1f, Enterobacteria_phage_k1_5, Enterobacteria_phage_lambda, Enterobacteria_phage_m13, Enterobacteria_phage_min27, Enterobacteria_phage_ms2, Enterobacteria_phage_mu, Enterobacteria_phage_n15, Enterobacteria_phage_n4, Enterobacteria_phage_p1, Enterobacteria_phage_p2, Enterobacteria_phage_p22, Enterobacteria_phage_p4, Enterobacteria_phage_phi1, Enterobacteria_phage_phieco32, Enterobacteria_phage_phiecom_gj1, Enterobacteria_phage_phip27, Enterobacteria_phage_phiv10, Enterobacteria_phage_phix174_sensu_lato, Enterobacteria_phage_prd1, Enterobacteria_phage_psp3, Enterobacteria_phage_rb14, Enterobacteria_phage_rb32, Enterobacteria_phage_rb43, Enterobacteria_phage_rb49, Enterobacteria_phage_rb51, Enterobacteria_phage_rb69, Enterobacteria_phage_rtp, Enterobacteria_phage_sf6, Enterobacteria_phage_sfv, Enterobacteria_phage_sp6, Enterobacteria_phage_ssl_2009a, Enterobacteria_phage_st104, Enterobacteria_phage_st64t, Enterobacteria_phage_st_1, Enterobacteria_phage_t1, Enterobacteria_phage_t3, Enterobacteria_phage_t4, Enterobacteria_phage_t5, Enterobacteria_phage_t7, Enterobacteria_phage_tls, Enterobacteria_phage_vt2_sakai, Enterobacteria_phage_wa13_sensu_lato, Enterobacteria_phage_wv8, Enterobacteria_phage_yyz_2008, Enterobacteriophage_qbeta

DISEASE(S): Not Available

SUBMITTER: Strader MB, et ak,  

PROVIDER: GPM11210013346 | GPMDB |

REPOSITORIES: GPMDB